Survivin和P27kip1在肝细胞癌中的表达及临床意义

目的 研究原发性肝癌中Survivin和P27kip1表达的关系,并探讨其在HCC中发生发展的作用.方法 收集HCC组织及相应癌旁组织标本各40例,应用免疫组织化学(SP)法,检测Survivin蛋白和P27kip1蛋白的表达.结果 85%(34/40)的肝癌组织表达Survivin蛋白,而癌旁及正常肝组织内无一例呈阳性表达.Survivin蛋白表达的阳性率在肝内转移者为96.7%,显著高于无肝内转移者 (50.0%,P<0.05);在门静脉癌栓浸润者为61.5%,显著高于无门静脉癌栓浸润者 (90.3%,P<0.05).20%(8/40)的肝癌组织内可见到P27kip1阳性表达,80%(32/40)癌旁组织可见不同程度P27kip1阳性表达,两者比较差异有统计学意义(P<0.05).34例Survivin表达阳性组织中有4例见P27kip1表达阳性 (11.7%),而6例 Survivin表达阴性组织中有4例见P27kip1阳性表达 (66.7%),两者比较差异统计学意义(P<0.05).结论 Survivin在肝癌组织中选择性表达,与肿瘤转移、血管浸润等恶性生物学行为有关,其作用机制可能涉及P27kip1蛋白阳性表达的降低,导致了细胞周期的失控并促进细胞异常增殖,从而参与了HCC的发生和发展.Abstract：Objective To investigate the role of Survivin and P27kip1 expression in the pathogenesis of hepatocellular carcinoma(HCC).Methods Forty histologically verified HCC specimens and their neighboring noncancerous tissues were obtained.Immunohistochemistry was used to detect protein expressions of Survivin and P27kip1.Results Survivin protein expression was positive in 34 of 40 (85%)cases.By comparison,no positive expression of Survivin was detected in corresponding tissues.The positive rate of Survivin protein expression in the cases of intrahepatic metastasis was higher than those without intrahepatic metastasis(P<0.05),and the rate of Survivin expression in the cases of portal vein invasion was higher than those without portal vein invasion(P<0.05).The expression of P27kip1 was positive in 8 of 40 (20%) cases and 80% in noncancerous tissues.The expression of P27kip1 was 11.7% in the cases of Survivin expression,which was lower than the cases without Survivin expression(66.7%).Conclusions The optional expression of Survivin have a close correlation with the tumor macastasis and vascular infiltration. Survivin expression may play an important part in HCC,which offer a wide prospective for clinical application in the primitive prognosis of hepatic-carcinoma.

Expression of Survivin and P27kip1 in hepatocellular carcinoma and its clinical significance

Hepatocellular carcinoma Survivin P27kip1