缩泉丸不同提取部位缩尿作用的谱效关系

目的:益智仁盐炙后组成缩泉丸,建立缩泉丸不同提取部位的指纹图谱,阐明各共有峰所代表的化学成分对缩尿药效的贡献大小,揭示其药效物质基础。方法:通过观察不同提取部位对单纯水负荷模型小鼠尿量系数的影响研究缩泉丸的缩尿药效,流动相乙腈-水梯度洗脱,检测波长230 nm,建立缩泉丸不同提取部位的HPLC指纹图谱,采用灰色关联分析法建立缩泉丸的谱效关系。结果:不同成分(共有峰编号)对缩泉丸缩尿作用贡献大小排序为8号9号7号11号17号5号19号29号18号10号6号12号23号。结论:建立的HPLC指纹图谱方法简便、重复性良好,可作为缩泉丸中益智仁盐炙后物质基础改变及药效改变的依据。初步证明缩泉丸的缩尿药效物质是一些极性较小的成分,且主要集中在石油醚部位,缩尿作用是这些成分协同作用的结果。

Spectrum-effect Relationship of Different Extract Parts of Suoquanwan Constituted with Salt-processed Alpiniae Oxyphyllae Fructus on Arresting Polyuria

Objective: To establish HPLC fingerprints of different extract parts of Suoquanwan constituted with salt-processed Alpiniae Oxyphyllae Fructus,to clarify the contribution of fingerprints peaks in different extract parts of Suoquanwan to reduce urination,and to reveal the pharmacodynamic material basis. Method: The influence of different extract parts on arresting polyuria of water-load mice was observed,HPLC fingerprints of different extract parts of Suoquanwan was established,grey relational analysis was adopted to establish spectrum-effect relationship. Result: Contribution of different peaks to reduce urination was ranked as No. 8 > No. 9 > No. 7 > No. 11 > No. 17 > No. 5 > No. 19 > No. 29 > No. 18 > No. 10 > No. 6 > No. 12 > No. 23. Conclusion: This HPLC fingerprints of different extract parts is simple with good reproducibility,it can be taken as a reference for quality evaluation of Suoquanwan.The pharmacodynamic substances are the low polar components,which concentrate in petroleum ether part,the effect of reducing urination is the result of synergistic action of those components.