| 响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性 |
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| 引用本文： | 杨立芳,刘洪存,支媛,郭洋洋,闫文超,姜明国,谭学才,石灵芳. 响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性[J]. 中国实验方剂学杂志, 2016, 22(23): 28-34 |
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| 作者姓名： | 杨立芳 刘洪存 支媛 郭洋洋 闫文超 姜明国 谭学才 石灵芳 |
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| 作者单位： | 广西民族大学 化学化工学院, 广西高校微生物与植物资源利用重点实验室, 广西高校食品安全与药物分析化学重点实验室, 南宁 530006,广西民族大学 化学化工学院, 广西高校微生物与植物资源利用重点实验室, 广西高校食品安全与药物分析化学重点实验室, 南宁 530006,广西民族大学 化学化工学院, 广西高校微生物与植物资源利用重点实验室, 广西高校食品安全与药物分析化学重点实验室, 南宁 530006,广西民族大学 化学化工学院, 广西高校微生物与植物资源利用重点实验室, 广西高校食品安全与药物分析化学重点实验室, 南宁 530006,中国药科大学药学院, 南京 210009,广西民族大学 海洋与生物技术学院, 南宁 530006,广西民族大学 化学化工学院, 广西高校微生物与植物资源利用重点实验室, 广西高校食品安全与药物分析化学重点实验室, 南宁 530006,广西民族大学 海洋与生物技术学院, 南宁 530006 |
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| 基金项目： | 国家自然科学基金项目（81560713）；广西教育厅科学研究项目（YB2014101） |
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| 摘 要： | 目的:利用响应曲面分析法优化毛果鱼藤总生物碱的提取工艺,并检测其抑菌活性。方法:以毛果鱼藤为原料,采用微波提取法,设计单因素实验,分别考察了料液比、乙醇体积分数、提取时间、微波提取功率、提取次数5个单因素对毛果鱼藤总生物碱提取效果的影响;采用响应曲面法优化毛果鱼藤总生物碱的提取工艺,提高总生物碱的提取效率,并考察总生物碱的体外抑菌作用。结果:毛果鱼藤总生物碱的最佳提取工艺条件为微波功率400 W,提取温度为70℃,料液比为1∶23,乙醇体积分数为70%,提取时间为23 min,在此条件下,以盐酸小檗碱计,总生物碱提取率为0.107 7 mg·g-1;抑菌实验表明,毛果鱼藤总生物碱对金黄色葡萄球菌有一定的抑菌作用。结论:微波法提取生物碱工艺方法科学、稳定,且生物碱具有良好的抑菌作用。
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| 关 键 词： | 毛果鱼藤 总生物碱 抑菌活性 响应曲面 |
| 收稿时间： | 2015-11-05 |
Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Derris eriocarpa Based on Response Surface Method |
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| YANG Li-fang,LIU Hong-cun,ZHI Yuan,GUO Yang-yang,YAN Wen-chao,JIANG Ming-guo,TAN Xue-cai and SHI Ling-fang. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Derris eriocarpa Based on Response Surface Method[J]. China Journal of Experimental Traditional Medical Formulae, 2016, 22(23): 28-34 |
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| Authors: | YANG Li-fang LIU Hong-cun ZHI Yuan GUO Yang-yang YAN Wen-chao JIANG Ming-guo TAN Xue-cai SHI Ling-fang |
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| Affiliation: | School of Chemistry and Chemical Engineering, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Guangxi University for Nationalities, Nanning 530006, China,School of Chemistry and Chemical Engineering, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Guangxi University for Nationalities, Nanning 530006, China,School of Chemistry and Chemical Engineering, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Guangxi University for Nationalities, Nanning 530006, China,School of Chemistry and Chemical Engineering, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Guangxi University for Nationalities, Nanning 530006, China,School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China,School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China,School of Chemistry and Chemical Engineering, Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Guangxi University for Nationalities, Nanning 530006, China and School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China |
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| Abstract: | |
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| Keywords: | Derris eriocarpa total alkaloids antibacterial activity response surface method |
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