HLA-DPB1基因rs9277542多态性与乙型肝炎病毒感染的关系

目的 探讨成都地区汉族人群中HLA-DPB1基因rs9277542位点多态性与HBV感染导致不同临床结局的关系.方法 收集成都地区非相关汉族人群的慢性HBV感染者579例（慢性HBV携带者222例,慢性乙型肝炎231例,乙肝肝硬化126例）和HBV自限感染者344例的外周血标本,应用SNapshot技术检测rs9277542位点基因型.结果 慢性HBV感染组与HBV自限性感染组rs9277542位点CC、CT、TT基因型和C、T等位基因频率分别为55.1％vs36.9％、38.3％vs48.0％、6.6％vs15.1％及74.3％vs60.9％、25.7％vs39.1％,两组比较差异有统计学意义（P＜0.0001）;以CC基因型和C等位基因为参照,CT、TT基因型和T等位基因比值比（OR）及95％可信区间（95％CI）分别为0.53（0.39-0.71）、0.28（0.18-0.46）、0.54（0.44-0.66）;乙肝肝硬化组与慢性HBV携带者组和慢性乙型肝炎组比较,CT＋ TT基因型频率分布差异有统计学意义（P=0.016,P=0.018）.结论 HLA-DPB1基因rs9277542多态性影响HBV感染后结局,其中T等位基因、TT基因型与HBV感染后自发清除相关,而携带XT基因型的慢性HBV感染者进展到乙肝肝硬化的风险更高.

Relationship Between Single Nucleotide Polymorphism of rs9277542 in HLA-DPB1 Gene and Different Outcomes of Hepatitis B Virus Infection

Objective To investigate the association of HLA-DPB1 variant （rs9277542） with the different clinical outcomes of HBV infection. Methods Blood samples were collected from 579 unrelated Chinese Han patients with persistent HBV infection, including 222 persistent asymptomatic HBV carriers （AsC）, 231 chronic active hepatitis B patients （CHB）, 126 HBV-related liver cirrhosis patients （LC） ,and 344 cases of spontaneously recovered HBV were enrolled as control. Snapshot technique was performed on genotyping rs9277542 polymorpbism in HLA- DPB1 gene. Results There was a significant difference in the allele and genotype frequencies of rs9277542 between persistent H BV infection group and control （all P〈0. 0001）. Taking CC genotype and C allele as reference, the odds ratio （OR） and 95% confidence intervals （95%CI） of the CT,TT genotypes and T allele were 0.53 （0.39-0.71）, 0.28 （0.18-0.46）,0.54 （0.44-0.66）, respectively. However, no significant differences were found in the genotype frequencies for CC,CT and TT between CHB group and AsC group （P：〉0.05）. Stratification analysis showed that the frequencies of TTq-CT genotype in LC group were significant higher than that in AsC group and CHB group （LC vs AsC：P=0. 016;LC vs CHB：P=0. 018,respectively）. Conclusion These findings indicate that rs9277542 variant in HLA-DPB1 gene could influence both the spontaneous clearance of HBV infection and the progression of patients with persistent HBV infection to HBV-related liver cirrhosis in the Han Chinese population.