云芝氯仿萃取物对肝癌HepG-2细胞的凋亡作用

目的]探讨云芝氯仿萃取物对肝癌HepG-2细胞的凋亡作用．方法]采用MTT方法检测不同浓度云芝氯仿萃取物对肝癌HepG．2细胞生存抑制率的影响；应用HE染色方法观察不同质量浓度云芝氯仿萃取物对肝癌HepG-2细胞形态学变化的影响．结果]MTT方法观察结果显示，云芝氯仿萃取物可抑制肝癌HepG-2细胞的增殖，随着时间的延长和药物浓度的增加，抑制作用越明显，当药物浓度为200mg／L、作用时间为48h时对HepG-2细胞的抑制率为52．3％，接近半数抑制浓度．HE染色结果显示，云芝氯仿萃取物处理的肝癌HepG．2细胞，与对照组相比细胞间距增大、细胞变小、失去原有形态、细胞核皱缩、染色变深、细胞碎裂、可见凋亡小体，并且随着时间的延长和剂量的增加，凋亡程度越严重．结论]云芝氯仿萃取物可促进肝癌HepG2细胞的凋亡．

Aapoptotic effect of coriolus versicolor chloroform extract on hepatocellular carcinoma HepG2 cells

OBJECTIVE To study the apoptotic effect of coriolus versicolor chloroform extract （CVCE） on hepatocellular carcinoma Hep-G2 cells in vitro. METHODS The viability inhibitory rate of different concentration of CVCE on HepG2 ceils was detected by MTT assay, and the effect of CVCE on HepG2 cell＇s morphologic changes was detected by HE staining. RESULTS The CVCE inhibited the proliferation of HepG2 cells, and the more were extension of time and increase of concentration, the more was its inhibitory effect. As 200 mg/L of CVCE concentration and 48 h of reaction time, the inhibition rate of HepG2 cells was 52.3 % which is closed to the half maximal inhibitory concentration （ICso）. As compared with the control group, HepG2 cells treated with CVCE were broadened cells interval, lessened size, crenated nuclear with darkened staining and cell fragmentation, and the apoptosis bodies were seen, and the apoptotic degree was more serious with the increasing of duration and drug concentration. CONCLUSION The CVCE can promote the apoptosis of hepatocellular carcinoma HepG2 cells in vitro.