| 芦丁对HepG2肝癌细胞AMPK蛋白信号通路的激活作用 |
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| 引用本文： | 元海丹,李海燕,王瑜玲,朴光春. 芦丁对HepG2肝癌细胞AMPK蛋白信号通路的激活作用[J]. 延边医学院学报, 2013, 0(3): 180-182 |
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| 作者姓名： | 元海丹 李海燕 王瑜玲 朴光春 |
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| 作者单位： | [1]延边大学药学院生药学教研部,吉林延吉133002 [2]牡丹江医学院红旗医院,黑龙江牡丹江157011 |
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| 基金项目： | 吉林省中医药管理局项目,项目编号：2012-156. |
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| 摘 要： | [目的]探讨芦丁在HepG2细胞中是否影响AMPK蛋白信号通路．[方法]采用MTS法测定细胞毒性；采用Westernblot方法检测AMPK蛋白的表达情况．[结果]芦丁在0-80μmol／L浓度范围内，在24h内对HepG2肝癌细胞没有毒性；芦丁呈浓度和时间依赖性地增高AMPK磷酸化．[结论]芦丁对HepG2肝癌细胞AMPK蛋白信号通路有激活怍用．
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| 关 键 词： | HepG2细胞 芦丁 AMPK |
Effects of rutin on activation of AMPK signal path in HepG2 cells |
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| YUAN Hai-dan,LI Hai-yan,WANG Yu-ling,PIAO Guang-chun. Effects of rutin on activation of AMPK signal path in HepG2 cells[J]. Journal of Medical Science Yanbian University, 2013, 0(3): 180-182 |
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| Authors: | YUAN Hai-dan LI Hai-yan WANG Yu-ling PIAO Guang-chun |
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| Affiliation: | 1 (1. Department of Pharmacognosy, Yanbian University College of Pharmacy, Yanji 133002, Jilin, China; 2. Hongqi Hospital of Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang, China) |
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| Abstract: | OBJECTIVE To study the effects of turin on activation of AMPK signal path in HepG2 human hepatoma cells. METHODS The toxicity of rulin for HepG2 cells was determined by MTS, and the expression of AMPK protein was determined by Western blot. RESULTS Ritin in concentration range of 0 to 80 μmol/L had no any cytotoxicity for HepG2 ceils, and turin enhanced AMPK phosphorylation in dose- and time-dependent manners. CONCLUSION Rutin can increase the activation of AMPK signal path in HepG2 human hepatoma cells. |
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| Keywords: | HepG2 cells Rutin AMPK |
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