Expression of epidermal growth factor receptor mRNA and protein in primary breast carcinomas |
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Authors: | Rosemary A. Walker Sheila J. Dearing |
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Affiliation: | (1) Breast Cancer Research Unit, University of Leicester, Clinical Sciences, Glenfield Hospital NHS Trust, Leicester, United Kingdom |
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Abstract: | The expression of epidermal growth factor receptor (EGFR) mRNA and protein has been determined in a group of breast carcinomas and compared to oestrogen and progesterone receptor (ER, PgR) status, as well as pathological features. In situ hybridization using a digoxigenin-labelled oligonucleotide probe was applied to formalin-fixed paraffin-embedded sections, and immunohistochemistry was used to determine EGFR protein.EGFR mRNA was detected in 66% of carcinomas with a third having labelling similar to normal breast tissue, 22% heterogeneous weak to strong labelling, and 11% strong labelling. EGFR protein was detected in 36% and these tumours had a strong correlation to lack of ER and high histological grade. The presence of EGFR protein was strongly correlated with more intense labelling for EGFR mRNA (p < 0.0001). This contrasted with normal breast in which both EGFR protein and mRNA were present with varying degrees in both tumours and a normal breast control. The ER-/PgR- carcinomas showed the full range of EGFR mRNA labelling. It is postulated that oestrogen or oestrogen regulated proteins are involved in regulation of EGFR mRNA and protein. In a proportion of tumours lacking steroid receptors regulation is lost, leading to EGFR overexpression. |
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Keywords: | breast cancer EGFR mRNA epidermal growth factor receptor immunohistochemistry in situ hybridization oestrogen receptor |
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