3, 3'-二氯联苯胺经口诱发A系小鼠实验肿瘤的观察

目的: 了解3,3'-二氯联苯胺(DCB)能否导致小鼠肿瘤发生率增高. 方法: A系小鼠经口喂饲不同剂量(64、 192、 576、 1 728 mg / kg)DCB 146 d,停药1个月后剖杀全部小鼠,重点检查肝、肺、肾的肿瘤发生情况,对肉眼可见的肿瘤进行病理学检查. 结果: 在A系小鼠肺脏和肝脏均有肿瘤发生,其中良性肿瘤包括肺腺瘤和肝胆管瘤,恶性肿瘤包括肺癌、肝胆管癌、肝细胞癌.最高剂量组的总肿瘤发生率和恶性肿瘤发生率分别为51.1 %和37.8 %,与对照组(17.6 %和0)相比,有统计学显著性(P<0.01, P<0.05). 结论: A系小鼠经口喂饲高剂量(136.2 mg / kg)DCB可致恶性肿瘤发生率增高.

EXPERIMENTAL NEOPLASIA IN STRAIN A MICE FOLLOWING ORAL ADMINISTRATION OF 3,3'-DICHLOROBENZIDINE

Purpose: To study whether 3,3'_dichlorobenzidine(DCB) c an increase the tumor incidence in strain A mice. Methods: S train A mice in 4 groups were orally administrated with DCB in 4 different dosages(64, 192, 576, 1 728 mg / kg) for 14 6 days. one month after stopping DCB, all the mice were ki lled and the viscera, especially the liver, lungs and kidneys were anatomically examined, and the tumors found in these or gans were further pathologically examined. Results: There were b enign tumors(lung adenoma, liver cholangioma) and malignant tumors (lung cancer, liver cholangiocarcinoma and hepatocellular carcinoma) in the lung and liver. The incidences of total tumors(51.1 %) and malignant tumors(37.8 %) of the higest dosage group were significantly higher than those of the control group(17. 6 %, 0)(P<0.01, P<0.05). Conclusion: DCB orally administrated at high dosage(136.2 mg / kg) can increase the malignant tumor i ncidence in strain A mice.