Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats |
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Authors: | Gustavo O Puntel Nélson R Carvalho Fernando Dobrachinski Andréia C F Salgueiro Robson L Puntel Vanderlei Folmer Nilda B V Barbosa Luiz F F Royes João Batista T Rocha Félix A A Soares |
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Institution: | 1. Departamento de Morfologia, Centro de Ciências de Saúde, Universidade Federal de Santa Maria, , Santa Maria, RS, Brasil;2. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, , Santa Maria, RS, Brasil;3. Universidade Federal do Pampa, UNIPAMPA – Campus Uruguaiana, , Uruguaiana, RS, Brasil;4. Departamento de Métodos e Técnicas Desportivas, Centro de Educa??o Física e Desportos, Universidade Federal de Santa Maria, , Santa Maria, RS, Brasil |
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Abstract: | The aim of this study was to analyze the effects of cryotherapy on the biochemical and morphological changes in ischemic and reperfused (I/R) gastrocnemius muscle of rats. Forty male Wistar rats were divided into control and I/R groups, and divided based on whether or not the rats were submitted to cryotherapy. Following the reperfusion period, biochemical and morphological analyses were performed. Following cryotherapy, a reduction in thiobarbituric acid‐reactive substances and dichlorofluorescein oxidation levels were observed in I/R muscle. Cryotherapy in I/R muscle also minimized effects such as decreased cellular viability, levels of non‐protein thiols and calcium ATPase activity as well as increased catalase activity. Cryotherapy also limited mitochondrial dysfunction and decreased the presence of neutrophils in I/R muscle, an effect that was corroborated by reduced myeloperoxidase activity in I/R muscle treated with cryotherapy. The effects of cryotherapy are associated with a reduction in the intensity of the inflammatory response and also with a decrease in mitochondrial dysfunction. |
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Keywords: | cryotherapy inflammatory response ischemia and reperfusion mitochondrial dysfunction oxidative stress |
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