原发性肝癌患者血清HBV标志物和HBV DNA测定的临床意义

目的观察原发性肝癌患者血清HBV标志物模式及HBV DNA水平。方法采用化学发光法检测151例PHC、132例慢性乙型肝炎和140例乙型肝炎肝硬化患者血清乙型肝炎病毒标记物;采用聚合酶链反应检测血清HBV DNA水平。结果在151例原发性肝癌患者中,HBV感染率达97.4%（147/151）,HBV DNA阳性率为74.8%（113/151）,平均水平为5.6±1.1 lgcopies/ml;HBsAg（＋）、HBeAb（＋）、HBcAb（＋）90例（59.6%）,其血清HBVDNA检出率为76.7%,平均水平为5.1±0.9lgcopies/ml;HBsAg（＋）、HBeAg（＋）、HBcAb（＋）38例（25.2%）,血清HBVDNA检出率100.0%,平均水平为6.4±0.9 lgcopies/ml。结论 PHC患者HBV感染率高,HBV与PHC发生有十分密切的关系。随着慢性乙型肝炎和乙型肝炎肝硬化病情的进展,血清HBeAg自发性发生血清学转换和HBV DNA载量下降,要警防原发性肝癌的发生。

Implications of serum hepatitis B viral DNA in patients with primary liver cancer

Objective To investigate the implication of serum hepatitis B viral HBV DNA in patients with primary liver cancer.Methods 151 patients with primary liverc cancer,132 patients with chronic hepatitis B,and 140 patients with liver cirrhosis were enrolled and serum markers of hepatitis B virus and HBV DNA were detected by using chemiluminescence and fluorescenct quantitative polymerase chain reaction.Results The serum markers of hepatitis B virus in 147 patients(97.35%) with primary liver cancer were positive,and serum HBV DNA positive rate was 74.8%(113/151) with an average load of 5.6±1.1 lgcopies/ml;in 90 patients(59.60%)with serum HBsAg,anti-HBe and anti-HBc positive,the serum HBV DNA positive rate was 76.7% with the average load of 5.1±0.9 lgcopies/ml,while in 38 patients(25.2%) with HBsAg,HBeAg and anti-HBc positive,the serum HBV DNA positive rate was 100.0% with the average HBV DNA load of 6.4±0.9 lgcopies/ml.Conclusion Hepatitis B virus infection rate was high in liver cancer patients,and it might play an important role in the hepatocarcinogenesis.