| 用毛细管气相色谱法快速分析鞘糖脂组分——介绍一种一次进样进行全组分分析的新方法 |
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| 引用本文： | 李瑞祥,朱正美,黄明贤,周良模.用毛细管气相色谱法快速分析鞘糖脂组分——介绍一种一次进样进行全组分分析的新方法[J].大连医科大学学报,1988(4). |
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| 作者姓名： | 李瑞祥 朱正美 黄明贤 周良模 |
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| 作者单位： | 大连医学院生化教研室，大连医学院生化教研室，中国科学院大连化物所，中国科学院大连化物所 |
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| 摘 要： | 本文提出了一种应用毛细管气相色谱对经过甲醇解及三甲基硅醚衍生化的鞘糖脂组分进行同时分析的方法。并应用本法对纯化的人肝细胞膜鞘糖脂 GM_3及人红细胞膜鞘糖脂 CTH 进行了分析,结果与该化合物已知组成相符。本法为鞘糖脂组分分析提供了快速、准确的方法,并可用于糖复合物中糖链的分析。
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| 关 键 词： | 鞘糖脂 三甲基硅醚衍生化 毛细管气相色谱分析 |
RAPID ANALYSIS OF GSL COMPONENTS BY A NEW CAPILLARY GC METHOD--INTRODUCING A NEW METHOD CHARACTERIZED BY A SINGLE INJECTION OF THE DERIVATIZED SAMPLE TO ANALYZE ALL COMPONENTS |
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| Li Ruixiang Zhu Zheng mei.RAPID ANALYSIS OF GSL COMPONENTS BY A NEW CAPILLARY GC METHOD--INTRODUCING A NEW METHOD CHARACTERIZED BY A SINGLE INJECTION OF THE DERIVATIZED SAMPLE TO ANALYZE ALL COMPONENTS[J].Journal of Dalian Medical University,1988(4). |
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| Authors: | Li Ruixiang Zhu Zheng mei |
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| Institution: | Li Ruixiang Zhu Zheng mei Department of Biochemistry,Dalian Medical College
Huang Mingxian Zhou Lianmo Dalian Institute of Chemical Physics,Academia Sinica |
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| Abstract: | The paper describes a rapid and effective capillary gaschromatography method for analyzing simultaneously components ofglycosphingolipids after methanolysis and trimethylsilation.Under sui-table temperature-programmed conditions,neutral hexoses,hexosamine(N-acetylgalactosamine,N-acetylglucosamine),N-acetyl neuraminicacid,sphingosine and fatty acids from purified glycosphingolipids GM_3and CTH were analyzed in one run with satisfactory accuracy throughthe application of the method. |
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| Keywords: | Glycosphingolipid Trimethylsilation Capillary GC Analysis |
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