杠柳根皮、茎皮中元素累积及其与活性成分相关性研究

目的测定不同采收期杠柳Periploca sepium根皮、茎皮中Mn、Cu、Zn、Fe、K、Ca、Mg量,探究元素累积变化规律及其与活性成分杠柳毒苷、4-甲氧基水杨醛的相关性,揭示影响2种成分累积的主导元素。方法电热板湿法消解,火焰原子吸收测定元素量,SPSS 19.0处理数据。结果 7种无机元素在根皮、茎皮中分布具有一定的差异性。根皮中Ca与杠柳毒苷,Fe与4-甲氧基水杨醛的累积趋势基本同步,茎皮中Fe与4-甲氧基水杨醛,Ca与Mg的累积趋势相近,呈"双峰"型。基于杠柳毒苷,在根皮中与Ca呈极显著正相关,在茎皮中与Zn呈显著负相关。基于4-甲氧基水杨醛,在根皮中与茎皮中4-甲氧基水杨醛、Fe呈极显著正相关,与茎皮中Mn呈显著负相关,与根皮中Fe呈显著正相关;在茎皮中与根皮、茎皮中Fe呈极显著正相关。结论 Ca、Zn、Fe、Mn是杠柳2种活性成分累积的关键元素,其中杠柳毒苷的主要影响元素为根皮Ca及茎皮Zn,间接影响元素为根皮Mg,4-甲氧基水杨醛的主要影响元素为Fe及茎皮Mn,茎皮Ca、Mg为其间接影响元素。

Correlation between elements accumulation and active components in velamen and stem bark of Periploca sepium

Objective To study the dynamic variation of Mn, Cu, Zn, Fe, K, Ca, and Mg in velamen and stem bark of Periploca sepium and their correlation with the elements such as periplocin and 4-methoxysalicylic aldehyde in different harvesting periods, and to reveal dominant factors on the accumulation of two active components. Methods The contents of periplocin and 4-methoxysalicylic aldehyde were detected by FAAS and electric heating wet digestion, and the data were analyzed by SPSS 19.0. Results The seven elements were varied widely in velamen and stem bark. The variation trends of Ca and periplocin, Fe and 4-methoxysalicylic aldehyde in velamen were similar, equally, those of Fe and 4-methoxysalicylic aldehyde, Ca and Mg in stem bark were similar and like double peaks. Periplocin has highly significant positive correlation with Ca in velamen and significant negative correlation with Zn in stem bark. To 4-methoxysalicylic aldehyde, when in velamen, it has highly significant positive correlation with 4-methoxysalicylic aldehyde and Fe in stem bark, significant negative correlation with Mn in stem bark, and significant positive correlation with Fe in velamen; when in stem bark, it has highly significant positive correlation with Fe in both velamen and stem bark. Conclusion Ca, Zn, Fe, and Mn are dominant elements of accumulation, the key factors for periplocin are Ca in velamen and Zn in stem bark, and Mg in velamen is indierect acting factors; The key factors for 4-methoxysalicylic aldehyde are Mn in stem bark and Fe, Ca and Mg in stem bark are indirect acting factors.