孤啡肽基因敲除小鼠电针镇痛作用增强

目的：采用痛敏肽/孤啡肽FQ (OFQ)基因敲除的小鼠，进一步明确OFQ在电针镇痛中的作用。方法：选用足三里和三阴交两个穴位，电针参数如下：恒流输出方波，电针强度为0.5、0.7、0.9 mA递增，每一强度持续10 min，电针频率分别为2 Hz或100 Hz。采用热辐射甩尾方法测定痛阈，在电针前、电针中和电针后评价痛阈的变化。结果：OFQ基因敲除小鼠的基础热痛阈比野生型小鼠明显延长。无论是2 Hz还是100 Hz电针，对OFQ基因敲除小鼠和野生型小鼠都有镇痛作用，对OFQ基因敲除小鼠的镇痛作用更强。结论：在体情况下，OFQ致痛敏，但拮抗电针镇痛。

Electroacupuncture analgesia is enhanced in transgenic nociceptin/orphanin FQ knock-out mice

Objective: The modulatory effects of nociceptin/orphanin FQ ( OFQ) on acupuncture ( EA, a odern version of acupuncture)-induced analgesia are still controversial. Transgenic OFQ knock-out mice rovide us a useful tool to investigate the role of endogenous OFQ in EA analgesia. The present tudy aims to investigate the role of OFQ in the EA-induced analgesia with OFQ knock-out mice. ethods; Acupoints were selected as "Zusanli" (ST36) and "Sanyinjiao" (SP6) , EA parameters were as ollows; constant current output, rectangular (square) wave pulses, increased intensities of 0. 5, 0. 7,0. 9 mA with stepwise fashion, 10 min for each intensity, frequency of 100 Hz (with 0. 2 ms as ulse width) or 2 Hz ( with 0. 6 ms as pulse width). Tail flick latency (TFL) evoked by radiant heat was used to evaluate the change of pain threshold before, during and after EA application. The nock-out mice were divided randomly into 3 groups; needling control, EA at 100 Hz and 2 Hz. ild-type mice of litter-mates of were used as Control. Results: It was found that OFQ knock-out mice had a longer basal ther-mal threshold; EA had enhanced analgesic effect in the knock-out mice than in wild-type Control mice. Conclusion: Endogenous OFQ might be algesic in basal condition and antagonize EA analgesia.