首页 | 本学科首页   官方微博 | 高级检索  
检索        

Akt对哮喘小鼠下呼吸道及内脏感觉传入部位IL-1β表达的影响
引用本文:张宝辉,刘玉丽,高鸣铎,于希富,张正洪,王平,方秀斌.Akt对哮喘小鼠下呼吸道及内脏感觉传入部位IL-1β表达的影响[J].解剖科学进展,2007,13(4):289-292.
作者姓名:张宝辉  刘玉丽  高鸣铎  于希富  张正洪  王平  方秀斌
作者单位:1. 中国医科大学基础医学院,神经生物学教研室,辽宁,沈阳,110001
2. 沈阳惠民生物有限公司,辽宁,沈阳,110141
3. 沈阳中药制药有限公司,辽宁,沈阳,110044
4. 郧阳医学院解剖教研室442000湖北,十堰市
基金项目:国家自然科学基金资助项目(No.30570811)
摘    要:目的探讨在哮喘发病机制中,PKB/Akt对哮喘小鼠肺组织及C7-T5段脊神经节和相应节段脊髓后角IL-1β表达的调节作用。方法BALB/c小鼠30只,按随机数字表法均分为正常对照组、哮喘组、PKB/Akt阻断组,免疫组织化学检测各组小鼠肺组织及C7-T5节段脊神经节和相应节段脊髓后角IL-1β的表达,Western blot方法检测各组小鼠肺组织及C7-T5节段脊神经IL-1β的表达。结果免疫组织化学结果显示,哮喘组肺、C7-T5段脊神经节和相应节段脊髓后角IL-1β阳性产物的平均光密度值(MOD)显著高于正常对照组(P<0.01);而PKB/Akt阻断组与哮喘组相比MOD值明显降低(P<0.05)。Western blot结果显示:与正常对照组比较,哮喘组小鼠肺、C7~T5段脊神经中IL-1β的IDV(integrated density value)与β-actin IDV的比值均明显升高(P<0.01),而PKB/Akt阻断组明显低于哮喘组(P<0.05)。结论NGF介导的Akt通路的激活可上调IL-1β的表达,介导哮喘气道高反应和炎症反应。

关 键 词:白介素1-β  PKB/Akt  神经生长因子  哮喘  下呼吸道  脊神经节  脊髓后角
文章编号:1006-2947(2007)04-0289-04
收稿时间:2007-10-25
修稿时间:2007年10月25

The Upregulatory Effect of PKB/Akt on IL -1β in the Lower Respiratory Tract and Viscerosensory Afferent Sites of the Asthmatic Mice
ZHANG Bao-hui,LIU Yu-li,GAO Ming-duo,YU Xi-fu,ZHANG Zheng-hong,WANG Ping,FANG Xiu-bin.The Upregulatory Effect of PKB/Akt on IL -1β in the Lower Respiratory Tract and Viscerosensory Afferent Sites of the Asthmatic Mice[J].Progress of Anatomical Sciences,2007,13(4):289-292.
Authors:ZHANG Bao-hui  LIU Yu-li  GAO Ming-duo  YU Xi-fu  ZHANG Zheng-hong  WANG Ping  FANG Xiu-bin
Abstract:Objective To study the regulatory effect of PKB/Akt on the expression of IL-1β in the lung, C7 - T5 spinal ganglia and the corresponding spinal dorsal horn of the asthmatic mice. Methods 30 BALB/c mice were randomly divided into normal control group, asthmatic group, the asthmatic mice blocked with LY294002 group on average. The expression of IL-1βwas observed by immunohistochemical method and Western blot. Results Immunohistochemistry showed that the mean optic density(MOD) of IL-1β positive product in the lung, spinal ganglia and dorsal horn of C7 - T5 increased significantly in the asthmatic mice than in normal control ( P < 0.01), but decreased in the asthmatic mice blocked with LY294002 than in the asthmatic mice ( P < 0.05). Western blot showed that the ratios of IL-1βintegrated density value ( IDV)/β-actin IDV in the lung, C7 - T5 spinal cord increased significantly in the asthmatic mice than in normal control ( P < 0.01), but decreased in the asthmatic mice blocked with LY294002 than in the asthmatic mice ( P < 0.05). Conclusion The upregulation of IL-1β by the activated NGF-mediated Akt pathway might be involved in the asthmatic airway hyperreactivity and inflammation.
Keywords:interleukin-1β  PKB/Akt  nervegrowth factor  asthma  lower respiratorytract  spinal ganglia  spinal dorsal horn
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号