L-NAME对内毒素致大鼠肺动脉内皮细胞损伤的保护作用

目的探讨左旋硝基精氨酸甲酯（L—NAME）是否对内毒素脂多糖（LPS）导致的大鼠肺动脉内皮细胞（RPAECs）损伤具有保护作用。方法利用大鼠肺动脉组织块贴壁法进行细胞原代培养。通过形态观察和Ⅷ因子相关抗原抗血清间接免疫荧光染色对细胞进行鉴定。将细胞分组，LPS、L—NAME与细胞共育24小时，检测各组细胞及培养上清中的硝基酪氨酸阳性细胞百分比（NT％）和乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）、内皮素-1（ET-1）的含量。结果L—NAME组中LDH（2237．04±50．32，2104．26±58．57）、MDA（8．36±0．25，7．26±0.24）比LPS组LDH（2553．01±55．75）、MDA（10．95±0．33）明显降低（P〈0．05），SOD（41．09±2．20，50．76±2．66）较LPS组SOD（33．16±2．9）明显升高（P〈0．05），NT％（33．7±1．94％，28．88±1．73％）与LPS组（40．28±2．3％）相比显著下降（P〈0．05），ET-1为（417．98±21．44，467．8±17．94Pg／ml，505．88±20pg／m1）与LPS组ET-1（391．67±14．62pg／m1）相比明显升高（P〈0．05）。结论L—NAME可能通过抗氧化作用来保护大鼠肺动脉内皮细胞。但是L—NAME升高细胞培养上清中的ET-1，单独用药可能导致肺动脉压力的增高。

Protection of L-NAME on RPAECs damage induced by LPS

Objective： To explore the protection of L-NAME in the RPAECS damage induced by LPS. Methods： ① Pulmonary artery endothelial cells were isolated and cultured by explant technique. ②The cells were identified through the observation of antigen anti - serum associated with Ⅷ factor by indirect immunofluorescence staining. ③The cells were randomly divided into three groups： the control group, the LPS group and the L-NAME group and cultured with LPS and L- NAME for 24 hours. ④ For the index detection, LDH, MDA, SOD, ET-1 and NT masculine percentage（NT% ） of every group＇s supernatant or cells were included. Results： Compared with the LPS group, the L-NAME group had lower LDH （2237.04 ± 50.32,2104.26±58.57 ）, MDA （ 8.36 ± 0.25,7.26 ± 0.24 ）, NT% （ 33.7 ±1.94%, 28.88 ± 1.73 % ）, ET-1 （417.98 ±21.44,467.8 ± 17.94 pg/ml,505.88 ±20 pg/ml） （P 〈0.05 ） and higher SOD（41.09 ±2.20,50.76 ±2.66 ） （P 〈 0. 05 ）. Conclusion ： L-NAME can lessen PAECs damage caused by LPS, which can be seen from the changes of LDH, MDA, SOD and NT%. However, ET-1 with the L-NAME rise in cell culture medium may lead to increased pul- monary artery pressur