金茵利胆灵对豚鼠胆石症及G蛋白偶联胆汁酸受体表达的影响

目的研究金茵利胆灵对豚鼠胆囊容积和成石率与G蛋白偶联胆汁酸受体表达的影响。方法取雌性豚鼠72只,随机分为6组,每组12只,除正常对照组外其余5组给予盐酸林可霉素皮下注射建立胆石症模型。治疗组分为4组给予阿司匹林,金茵利胆灵高、中、低剂量药液灌喂,末次给药后1 h,切除胆囊测量胆囊容积,计算每组动物的胆囊内成石率。将胆囊组织进行HE染色后行显微镜观察,并行免疫组化观察阳性细胞。多组间比较采用单因素方差分析,两组间比较用LSD-t法;计数资料采用χ2检验,两组间比较调整检验水准进行比较;等级资料采用Kruskal-Wallis H检验。结果模型组成石率高于正常对照组,差异有统计学意义(P=0.00);金茵利胆灵高剂量组成石率较其他组降低。模型组胆囊大量中性粒细胞浸润,炎性改变明显;各药物治疗组中金茵高剂量组炎性改变最轻。模型组胆囊容积增加明显,与正常对照组相比差异有统计学意义(P0.01),造模效果较好;金茵利胆灵高剂量组胆囊容积相对最小,金茵利胆灵中剂量组和阿司匹林组胆囊容积相对略小,与模型组相比差异均有统计学意义(P0.01)。模型组胆囊黏膜TGR5阳性表达强,与正常对照组相比差异有统计学意义(P=0.00);金茵利胆灵高剂量组黏膜TGR5阳性表达较弱,与模型组相比差异有统计学意义(P0.01)。结论 TGR5在胆石症豚鼠胆囊黏膜的表达显著增强。金茵利胆灵可以降低胆石症豚鼠胆囊黏膜TGR5阳性表达率,可减轻胆囊炎症,减少胆石形成。

Effects of Jinyinlidanling on cholelithiasis and expression of G protein-coupled bile acid receptor in guinea pigs

Objective To investigate the effects of Jinyinlidanling （JYLDL）on gallbladder volume,rate of gallstone formation,and the ex-pression of G protein-coupled bile acid receptor （TGR5 ）in guinea pigs.Methods Seventy-two female guinea pigs were randomly divid-ed into treatment groups,normal control group,and model group,with 12 ones in each group.Guinea pigs in the treatment groups and mod-el group were subcutaneously injected with lincomycin hydrochloride to build a cholelithiasis model,while the normal control group with the same volume of normal saline.The treatment groups were divided into four groups which were separately given aspirin and low-,medium-and high-dose JYLDL.At one hour after the last administration,the gallbladder of each guinea pig was resected to measure the gallbladder volume and calculate the rate of gallstone formation.HE staining was applied for microscopic observation,and positive cells （with expression of TGR5 ）were detected by immunohistochemistry.Comparison between multiple groups was made by one-way ANOVA,and comparison between two groups by LSD-t test;comparison of categorical data was made by chi-square test,and the significance level was adjusted when comparison was made between two groups;comparison of ranked data was made by Kruskal Wallis H test.Results The rate of gall-stone formation in the model group was significantly higher than that in the control group （P=0.00）;the rate of gallstone formation in the high-dose JYLDL group was lower than those of other groups.Typical inflammatory changes and infiltration of neutrophils were observed in the model group;the high-dose JYLDL group had the mildest inflammatory changes among treatment groups.The gallbladder volume of the model group was significantly bigger than that of the control group （P〈0.01）,indicating that the cholelithiasis model was successfully es-tablished;the gallbladder volume of the high-dose JYLDL group was the smallest and those of the medium-dose JYLDL group and aspirin group were smaller,as compa