Cellular Ca2+ Dynamics in Urinary Bladder Smooth Muscle From Transgenic Mice Overexpressing Na+-Ca2+ Exchanger |
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| Authors: | Hidemichi Murata Shingo Hotta Eiji Sawada Hisao Yamamura Susumu Ohya Satomi Kita Takahiro Iwamoto Yuji Imaizumi |
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| Institution: | 1. Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan;2. Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan |
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| Abstract: | The rise of Ca2+ concentration (Ca2+] i ) by reducing external Na+ in urinary bladder smooth muscle cells (UBSMCs) from transgenic mice overexpressing Na+/Ca2+ exchanger type-1.3 (NCX1.3tg/tg) was about 4 times as large as that in the wild-type (WT). NCX1 protein expression in UB increased about 4-fold in NCX1.3tg/tg. The Ca2+ release by caffeine in UBSMCs was comparable between NCX1.3tg/tg and WT, but Ca2+]i decay was faster in NCX1.3tg/tg. Contractions induced by acetylcholine, 60 mM K+, or electrical stimulation were significantly smaller in UB segments of NCX1.3tg/tg. NCX worked in Ca2+-extrusion mode during these contractions in UBSMCs of both WT and NCX1.3tg/tg. |
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| Keywords: | urinary bladder smooth muscle transgenic mouse |
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