Sequence polymorphism of the mitochondrial DNA control region in the population of Vojvodina Province,Serbia |
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Authors: | Dragana Zgonjanin Igor Veselinovi? Milovan Kubat Ivana Fura? Mirjana Antov Eva Lon?ar Milo? Tasi? Radenko Vukovi? Radovan Omorjan |
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Institution: | 1. Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Republic of Serbia;2. Department of Forensic Medicine and Criminology, University of Zagreb School of Medicine, Zagreb, Croatia;3. University of Novi Sad, Faculty of Technology, Department of Applied Chemistry, 21000 Novi Sad, Bul. Cara Lazara 1, Serbia |
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Abstract: | In order to generate and establish the database for forensic identification purposes in Vojvodina Province (Serbia), the sequence of the hypervariable regions 1 (HV1) and 2 (HV2) of the mtDNA control region were determined in a population of 104 unrelated individuals from Vojvodina Province, using a fluorescent-based capillary electrophoresis sequencing method. A total of 93 different haplotypes were found, of these 83 mtDNA types were unique, nine haplotypes were shared by two individuals and one haplotype by three individuals. The variation of mtDNA HV1 and HV2 regions was confined to 116 nucleotide positions, of which 72 were observed in the HV1 and 44 in the HV2. A statistical estimate of the results for this population showed the genetic diversity of 0.9977 and the random match probability of 1.18%. Haplogroup H was the most common haplogroup (43.3%). Haplogroups observed at intermediate levels included clusters U (13.5%), T (10.6%), J (8.6%) and W (5.8%). |
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