miR-411-5p对胃癌细胞凋亡、增殖和干样特性以及裸鼠成瘤的影响

目的 miR-411-5p对胃癌SGC-7901细胞生物学特性及裸鼠成瘤的影响.方法 荧光定量PCR(RT-qPCR)检测转染效率.5-溴脱氧尿嘧啶核苷(BrdU)染色检测增殖.流式细胞术检测凋亡.干细胞成球实验检测细胞干样特性.蛋白印迹实验检测增殖细胞核抗原(PCNA)、凋亡抑制蛋白(Survivin)、半胱天冬酶-...

Effects of miR-411-5p on Apoptosis,Proliferation,Stemness Characteristics of Gastric Cancer Cells and Tumor Formation in Nude Mice

Objective To investigate the effect of Mir-411-5p on the biological characteristics of gastric cancer SGC-7901 cell and tumor formation in nude mice.Methods RT-qPCR was used to detect transfection efficiency.BrdU staining was used to detect proliferation.Flow cytometry was used to detect apoptosis.Stem cell pellet-forming assay was used to detect the characteristics of dry cells.The expression of PCNA,Survivin,Caspase-3,Caspase-9 and P21 proteins were detected by western blot.The expression of PCNA and Caspase-3 in tumor tissues was detected by immunohistochemical assay.Results Compared with the control group,when miR-411-5p overexpressedBrdU positive cells were decreased,and PCNA and Survivin protein expression were down-regulated.The apoptosis rate was decreased,and the expressions of caspase-3,Caspase-9 and P21 were increased.The diameter of stem cells into spheres decreased,and the number of spheres decreased.Compared with miR-NC group,tumor mass and tumor volume decreased.The expression of PCNA and Caspase-3 in tumor tissues was down-regulated.Conclusion The overexpression of miR-411-5p can inhibit the proliferation and stem-like characteristics of gastric cancer SGC-7901 cells,promote cell apoptosis,and inhibit the growth of xenografts in nude mice.