Induction of antibodies by Staphylococcus aureus nasal colonization in young children |
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Authors: | NJ Verkaik A Lebon CP de Vogel H Hooijkaas HA Verbrugh VWV Jaddoe A Hofman HA Moll A van Belkum WJB van Wamel |
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Institution: | 1. Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands;2. Department of Paediatrics, Erasmus MC–Sophia Children''s Hospital, Rotterdam, The Netherlands;3. The Generation R Study Group, Rotterdam, The Netherlands;4. Department of Immunology, Rotterdam, The Netherlands;5. Department of Epidemiology, Erasmus MC, Rotterdam, The Netherlands |
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Abstract: | In order to develop novel antlstaphylococcal strategies, understanding the determinants of carriage and how humans respond to Staphylococcus aureus exposure is essential. Here, the primary S. aureus-specific humoral immune response and its association with nasal colonization was studied in young children. Sera from 57 colonized or non-colonized children, serially collected at birth and at 6, 14 and 24 months, were analysed for IgG, IgA and IgM binding to 19 staphylococcal proteins, using flow cytometry-based technology. The antibody responses showed extensive inter-individual variability. On average, the levels of antistaphylococcal IgA and IgM increased from birth until the age of 2 years (p <0.05), whereas the levels of IgG decreased (p <0.001). Placentally transferred maternal IgG did not protect against colonization. In colonized children, IgG and IgA levels for a number of proteins were higher than in non-colonized children. At both 14 and 24 months, the levels of IgG against chemotaxis inhibitory protein of S. aureus (at 24 months; median fluorescence intensity, 4928 vs. 24, p <0.05), extracellular fibrinogen-binding protein (987 vs. 604, p <0.05), and iron-responsive surface determinant H (62 vs. 5, p <0.05) were significantly higher in colonized children. The levels of IgA against CHIPS, IsdH and IsdA were higher (p <0.05). Therefore, CHIPS, Efb, IsdA and IsdH seem to play a role in nasal colonization of young children. |
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Keywords: | Antibodies children colonization luminex |
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