首页 | 本学科首页   官方微博 | 高级检索  
检索        

复可托产品及原料中6种猪病毒基因膜芯片检测方法研究
引用本文:洪亮,王福云,潘映秋,卢敏.复可托产品及原料中6种猪病毒基因膜芯片检测方法研究[J].中国现代应用药学,2022,39(23):3110-3116.
作者姓名:洪亮  王福云  潘映秋  卢敏
作者单位:台州市药品检验研究院, 浙江 台州 318000;浙江丰安生物制药有限公司, 浙江 台州 318000
基金项目:台州市科技计划项目(20ywb109)
摘    要:目的 利用基因膜芯片技术建立复可托产品及原料中6种猪病毒快速检测方法。方法 设计6种猪病毒特异性引物及探针,对样本核酸进行多重PCR扩增,进一步利用基因膜芯片技术进行产物杂交并分析判定。结果 建立的检测方法特异性强,稳定性好,灵敏度高,综合检测限为1.0×105 copies·mL-1,满足日常检验要求。结论 建立了6种猪病毒PCR-基因膜芯片技术的快速筛查方法,可保障复可托产品及原料的质量安全,该技术可进一步应用于养殖场和市场的猪肉检测,为政府监管部门提供有力的技术支撑。

关 键 词:复可托  猪病毒  基因膜芯片  多重PCR
收稿时间:2022/1/28 0:00:00
修稿时间:2022/10/16 0:00:00

Study on Detection Method of Six Porcine Viruses in Fucotto Products and Raw Materials by Gene Membrane Chip
HONG Liang,WANG Fuyun,PAN Yingqiu,LU Min.Study on Detection Method of Six Porcine Viruses in Fucotto Products and Raw Materials by Gene Membrane Chip[J].The Chinese Journal of Modern Applied Pharmacy,2022,39(23):3110-3116.
Authors:HONG Liang  WANG Fuyun  PAN Yingqiu  LU Min
Institution:Taizhou Institute for Drug Control, Taizhou 318000, China;Zhejiang Feng''an Biopharmaceutical Co., Ltd., Taizhou 318000, China
Abstract:OBJECTIVE To establish a rapid detection method of six porcine viruses in fucotto products and raw materials by using gene membrane chip technology. METHODS Six specific primers and probes of porcine viruses were designed to amplify the sample nucleic acid by PCR, and the products were hybridized and analyzed by gene membrane chip technology. RESULTS The established detection method had strong specificity, good stability and high sensitivity. The comprehensive detection limit was 1.0×105 copies·mL-1, which meet the requirements of daily inspection. CONCLUSION The rapid screening method of PCR gene membrane chip technology for six porcine viruses has been established, which can ensure the quality and safety of fucotto products and raw materials. This technology can be further applied to the detection of pork in farms and markets, and provide strong technical support for government regulators.
Keywords:fucotto  porcine virus  gene membrane chip  multiplex PCR
点击此处可从《中国现代应用药学》浏览原始摘要信息
点击此处可从《中国现代应用药学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号