阿萨伊对虚热和虚寒证大鼠的脂肪代谢、免疫物质和内分泌激素的影响

研究阿萨伊对虚热证、虚寒证大鼠的脂肪代谢、免疫物质及内分泌激素水平变化。SD大鼠分为空白组、虚热模型组、虚热模型加黄柏组、虚热模型加阿萨伊高、低剂量组、虚寒模型组、虚寒模型加肉桂组、虚寒模型加阿萨伊高、低剂量组;采用每日肌肉注射地塞米松磷酸钠、氢化可的松琥珀酸钠1次、连续21 d的方法制备大鼠虚热模型、虚寒模型;通过比色法检测脂肪代谢(FFA,LPL,HL)指标变化以及免疫(IgG,IgM,C3,C4)指标变化;通过放免法检测内分泌激素(CORT,E2,T)指标变化。阿萨伊高剂量组能降低虚热证大鼠血清中FFA,LPL,HL值(P0.01或P0.001);阿萨伊高剂量组能显著升高虚热证大鼠血清中IgG,IgM,C3值(P0.05或P0.001);阿萨伊高剂量组能显著升高虚热证大鼠血清中CORT值(P0.05),能显著降低E2,E2/T值(P0.05);阿萨伊高剂量组对虚寒模型脂肪代谢指标作用不明显,但可以升高免疫指标IgG,IgM,C3值(P0.05或P0.001),同时对内分泌激素指标CORT值也有升高的作用(P0.05)。阿萨伊的作用趋向与黄柏一致,表现出对虚热模型大鼠的调整作用;与肉桂相反,对虚寒模型大鼠的指标纠正不明显。本实验从同类比较、异类反证角度验证了理论推导的阿萨伊中药寒凉药性。

Effect of Açaí (Euterpe oleracea) on lipid metabolism, immune substances and endocrine hormone in rats with deficiency-heat and deficiency-cold syndrome

To study the effects of Açaí (Euterpe oleracea) on lipid metabolism, immune substances and endocrine hormone level in rats with deficiency-heat and deficiency-cold syndrome. SD rats were divided into blank control group, deficiency-heat model group, deficiency-heat & Phellodendri Cortex group, deficiency-heat & Açaí high dose and low dose groups, deficiency-cold model group, deficiency-cold & Cinnamomi Cortex group, deficiency-cold & Açaí high dose and low dose groups. The rats received intramuscular injection of dexamethasone sodium phosphate (0.35 mg) or hydrocortisone sodium succinate (20 mg) for 21 days to set up deficiency-heat models and deficiency-cold models. Then the changes in fatmetabolism levels (FFA, LPL, HL) and immune indexes (IgG, IgM, C3 and C4) were detected by colorimeter; and the levels of endocrine hormone indexes (CORT, E2 and T) were detected by radioimmunoassay. The levels of FFA, LPL and HL in serum were reduced (P<0.01 or P<0.001); levels of IgG, IgM and C3 in serum were increased (P<0.05 or P<0.001); level of CORT in serum was increased (P<0.05) and the level of E2, E2/T in serum were reduced in the Açaí high dose group (P<0.05). The effect of high dose Açaí on fat metabolism was not obvious in deficiency-cold models, but the levels of IgG, IgM, C3 and CORT in serum were increased (P<0.05 or P<0.001). Açaí was showed the same effect trend with Phellodendri Cortex in adjusting the levels of deficiency-heat rats; but unlike Cinnamomi Cortex, Açaí was showed no obvious effect in adjusting the levels of deficiency-cold rats. In this experiment, homogeneous comparison and heterogeneous disproof were used to verify the cold nature of Açaí.