胃癌中CXCR4 mRNA的表达量及其临床意义

摘 要：［目的］ 探究胃癌组织中与胃癌进展相关的趋化因子受体4（C-X-C motif chemokine receptor 4，CXCR4）的表达量及其临床意义。［方法］ 先根据TCGA数据库，对300余例患者胃癌组织及癌旁组织的CXCR4 mRNA表达量进行差异性分析，并与病理参数进行相关性分析。再使用逆转录聚合酶链式反应（RT-PCR）和免疫组化（immunohistochemistry，IHC）技术检测2018年7月至2022年11月陕西省人民医院收治的92例患者胃癌组织标本中CXCR4 mRNA的表达量并分析其与预后和病理参数的相关性。［结果］ TCGA数据及临床数据分析均显示，胃癌组织的CXCR4 mRNA表达水平显著高于癌旁组织（P＜0.001）。TCGA数据分析显示，CXCR4 mRNA表达量与肿瘤浸润深度及低分化程度呈正相关（OR=1.297、1.437，P均＜0.05）。临床数据分析显示，CXCR4 mRNA表达量与肿瘤远处转移及分化程度呈正相关（OR=7.717、3.254，P均＜0.05）。TCGA数据及临床数据Kaplan-Meier生存分析均显示，与CXCR4 mRNA低表达组相比，高表达组胃癌患者生存时间明显缩短（P=0.008、0.001）。［结论］ 胃癌组织中CXCR4 mRNA过表达，与肿瘤的进展、浸润、转移密切相关，其高表达提示胃癌患者的不良预后。CXCR4 mRNA可能成为胃癌诊断和靶向治疗新的候选标志物。

Expression of CXCR4 mRNA in Gastric Cancer and Its Clinical Significance

Abstract：［Objective］ To investigate the expression of C-X-C Motif chemokine receptor 4 (CXCR4) in gastric cancer and its clinical significance. ［Methods］ The data of CXCR4 mRNA expression in cancer tissues and pericancerous tissues， and pathological parameters of gastric cancer patients were obtained from TCGA database. The expression of CXCR4 mRNA in cancer tissue specimens of 92 gastric cancer patients who underwent gastrectomy in Shaanxi Provincial People’s Hospital between July 2018 and November 2022 was detectedby RT-PCR and immunohistochemistry. The correlation of CXCR4 mRNA expression with pathological parameters and prognosis of patients was analyzed. ［Results］ Both TCGA data and clinical data showed that CXCR4 mRNA expression levels in gastric cancer tissues were significantly higher than those in pericancerous tissues (P＜0.001). TCGA data analysis showed that CXCR4 mRNA expression was positively correlated with tumor infiltration depth (T) and degree of tumor differentiation (OR=1.297 and 1.437， P＜0.05). Clinical data analysis showed that CXCR4 mRNA expression was positively correlated with distant tumor metastasis and the degree of tumor differentiation (OR=7.717 and 3.254，P＜0.05). Both TCGA data and clinical data showed that the survival time of gastric cancer patients with high-expression of CXCR4 mRNA was significantly shorter those with low-expression of CXCR4 mRNA (P=0.008 and 0.001). ［Conclusion］ CXCR4 mRNA overexpression in gastric cancer tissues is closely related to tumor development， infiltration， and metastasis， and indicates a poor prognosis of patients. CXCR4 mRNA might be a new candidate marker for diagnosis and potential target for treatment in gastric cancer.