| HPLC法测定刺五加果实中异嗪皮啶的含量 |
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| 引用本文： | 张海丰,滕坤,朱俊义.HPLC法测定刺五加果实中异嗪皮啶的含量[J].中国药物警戒,2010,7(9):513-514. |
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| 作者姓名： | 张海丰 滕坤 朱俊义 |
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| 作者单位： | 通化师范学院,吉林,通化,134000 |
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| 基金项目： | 刺五加果醋生产工艺研究;吉教科合字[2008]第215号，100吨刺五加果实综合开发技术研究;吉教科合字[2009]第265号 |
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| 摘 要： | 目的建立HPLC测定刺五加果实中异嗪皮啶含量的方法。方法采用AgilentXDB-C18色谱柱（4.6mm×150mm,5μm）,以甲醇-0.2%磷酸溶液（27：73）为流动相,流速为1.0mL·min-1,柱温为室温,进样量10μL,检测波长为343nm。结果异嗪皮啶进样量在0.334～4.175μg·mL-1内呈良好的线性关系,r=0.9996,平均加样回收率为100.74%,RSD为0.44%（n=6）。结论该方法灵敏快速、结果准确、重现性好,可用于刺五加果实中异嗪皮啶含量的测定。
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| 关 键 词： | 刺五加果实 异嗪皮啶 高效液相色谱 |
Determination of Isofraxidin in Medofenoxate Fruits by HPLC |
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| ZHANG Hai-feng,TENG Kun,ZHU Jun-yi.Determination of Isofraxidin in Medofenoxate Fruits by HPLC[J].Chinese JOurnal of Pharmacovigilance,2010,7(9):513-514. |
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| Authors: | ZHANG Hai-feng TENG Kun ZHU Jun-yi |
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| Institution: | (Tonghua Normal University,Jilin Tonghua,134000,China) |
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| Abstract: | Objective To establish a HPLC method for the determination of isofraxidin in medofenoxate fruits.Methods Agilent XDB C18（4.6 mm×150 mm,5μm） column was applied,using methanol-0.2% phosphoric acid （27：73）as mobile phase with the rate of 1.0mL·min-1.The column temperature was room temperature,the injection volumn was 10μL and the detective wavelength was 343 nm.Results A good linearity was obtained over the range of 0.835～4.176μg·mL-1 for isofraxidin（r=0.9996）.The average recovery（n=6） was 100.74%（RSD=0.44%）.Conclusion The method is practical,convenient,accurate,and can be used as the determination of isofraxidin in medofenoxate fruits. |
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| Keywords: | medofenoxate fruit isofraxidin HPLC |
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