胞二磷胆碱预处理对大鼠脊髓缺血再灌注损伤的影响

目的 探讨胞二磷胆碱对脊髓缺血再灌注损伤的影响及其作用机制.方法 雄性SD大鼠72只,体质量(230±20)g,按随机数字表法分为3组(n=24):假手术组,缺血再灌注损伤组(IR组)和胞二磷胆碱预先给药组(citi组),各组按灌注后12、24、48、72 h分成4个亚组(n=6).各组均采用改良Zivin法建立脊髓...

Citicoline pretreatment protects rats against spinal cord ischemia-reperfusion injury

Objective To determine the effects of citicoline pretreatment on spinal cord ischemia-reperfusion(IR) injury in rats.Methods Seventy-two male SD rats weighing 210 to 250 g were randomly divided into 3 groups(n=24): sham operation group,ischemia-reperfusion injury group(group IR) and citicoline pretreatment group(group citi).Every group was divided into 4 subgroups according to the time after reperfusion for 12,24,48 and 72 h respectively(n=6).After modified Zivin methods was used to establish model,Tarlov with modification was used for neurological function scores at the above mentioned time points,HE staining was employed for L2-5 spinal cord pathological changes,TUNEL method for calculation apoptosis cells,and immunohistochemical assay for Bcl-2,Bax,caspase-9 expression.The ratio of Bcl-2 to Bax(Bcl-2/Bax) was calculated.Results Compared to Sham group,group IR and group citi showed significantly lower neurological function scores,obvious pathological injury,increased apoptotic nerve cells,enhanced expression of caspase-9,Bax and Bcl-2,and significantly lower Bcl-2/Bax ratio(P<0.01).However,citi pretreatment resulted in better outcomes in above items compared to group IR(P<0.01).Conclusion Citicoline pretreatment attenuates spinal cord IR injury,improves neurological function through up-regulating Bcl-2,down-regulating Bax and caspase-9,and thus reducing neuronal apoptosis.