葡萄糖-6-磷酸脱氢酶对皮层注射FeCl3所致早期癫痫的保护作用

目的 探讨葡萄糖-6-磷酸脱氢酶(glucose.6-phosphate dehydrogenase,G6PD)在外伤后早期癫痫中的脑保护作用.方法 采用大脑皮层微量注射FeCl3溶液建立癫痫大鼠动物模型.将24只SD大鼠抽签随机分为对照组、实验组、干预组(n=8),对照组和实验组腹腔注射灭菌花生油10 ml/kg,干...

Glucose-6-phosphate dehydrogenase protects early epilepsy in rats after intracortical injection of ferric chloride

Objective To determine the cerebral protective effect of glucose-6-phosphate dehydrogenase(G6PD) in rat early epilepsy.Methods Twenty-four SD rats were randomly and equally divided into 3 groups,normal control,model group and intervention group.The rats of intervention groups received an intraperitoneal injection of 0.1 μg/ml 1,25-dihydroxyvitamin D3(1,25-(OH)2D3) for 7 d,and the animals of the normal control and model group were administered with an intracortical injection of normal saline.Rat models of epilepsy were established by intracortical microinjection of ferric chloride and identified by electroencephalography(EEG) in 2 h later and occurrence of seizures by behavior observation in 6 h later.Expression of G6PD mRNA and protein were detected in the cerebral cortex by RT-PCR and Western blotting.Malondialdehyde(MDA) level of lipid peroxidation and antioxidant level of glutathione(GSH) and G6PD activity were detected by colorimetric method.Results Typical seizures were observed in model and intervention groups.The seriousness and duration of epilepsy seizures of rats from intervention group were less obvious than those of model groups(P<0.05).The expressions of G6PD mRNA and protein in injection zone of cortex from intervention groups were higher than those in the model groups(P<0.05),but lower than those in the control groups(P>0.05).The G6PD activity and GSH level were increased(P<0.05) but the MDA level was significantly reduced in the intervention groups(P<0.05).Conclusion G6PD can improve the antioxidant capacity of brain cortex and protect rats from the FeCl3-induced epilepsy.