呼吸道合胞病毒感染患儿血清嗜酸性粒细胞趋化蛋白测定的临床意义

目的探讨呼吸道合胞病毒(RSV)感染患儿血清嗜酸性粒细胞趋化蛋白(eotaxin)的表达与呼吸道高气道反应的相关性。方法收集40例RSV感染患儿的血清,其中27例为哮喘急性发作患儿(哮喘组),13例为喘息性支气管炎(喘支组),采用双抗体夹心酶联免疫吸附试验(ELISA)方法测其血清eotaxin的含量,并与正常对照组比较。结果哮喘组与喘支组及正常对照组的eotaxin含量比较均有显著性意义(P<0.05)。血清eotaxin的水平与外周血嗜酸性粒细胞(Eos)总数呈正相关(r=0.4196,P<0.001)。结论RSV感染后血清趋化因子表达增加,导致气道高反应是RSV感染致哮喘的病理基础。

The Clinical Significance of Detection of Eotaxin in Serum of Infants with Respiratory Syncytial Virus Infection

Objective To explore the correlation of the concentrations of serum eotaxin in serum and airway hyperresponsiveness in the pathogenesis of respiratory syncytial virus(RSV) infection. Methods Serum amples were obtained from 40 infants with RSV infection,including 27 acute asthma , 13 asthmatic bronchitis. ELISA method was used to detemine to concentrations of eotaxin in serum. Results eotaxin levels of acute asthma in infants with RSV infection were much higher than that of asthmatic bronchitis and those of non- RSV infected healthy subjects (P <0.05), and the levels of eotaxin in asthmatic bronchitis were higher than infection the normal group. It also found that serum concentrations of eotaxin were positively correlated to the number of eosinophils(r =0.4196, P <0.001). Conclusion The concentrations of chemokines in serum will increasing after infection of respiratory syncytial virus , leading to airway hyperresponsiveness, it is the pathological base from RSV infection on bronchitis asthma.