外周血幽门螺杆菌DNA的检测

目的　探讨幽门螺杆菌是否可导致菌血症。方法　采用聚合酶链反应(PCR)对2 0例消化性溃疡及慢性胃炎患者的外周血和胃黏膜组织进行幽门螺杆菌DNA检测,并对胃黏膜组织进行幽门螺杆菌分离培养。结果　在2 0个病例中,9例胃黏膜组织分离培养出了幽门螺杆菌,15例胃黏膜组织PCR扩增出幽门螺杆菌DNA ;2 0份外周血标本中有3份PCR扩增出幽门螺杆菌DNA ,其16SrRNA、cagA基因为阳性,该3例患者的胃黏膜组织也扩增出了幽门螺杆菌DNA ,并分离培养出幽门螺杆菌。结论　幽门螺杆菌不仅存在于胃黏膜组织中,也可存在于血液中,提示幽门螺杆菌可引起菌血症。

Detection of Helicobacter pylori DNA in peripheral blood

The aim of the present study is to search for the possibility of bacteremia caused by Helicobacter pylori. H. pylori DNA in peripheral blood and gastric mucosa of 20 patients,with peptic ulcer or chronic gastritis, was examined by polymerase chain reaction (PCR), and in addition, H. pylori in gastic mucosa was cultivated . Of 20 patients, 9 were positive for H. pylori in cultures, 15 were positive for the amplification of H. pylori DNA in gastric mucosa and 3 were positive for the amplification of H. pylori DNA in peripheral blood, H. pylori DNA in gastric mucosa and H. pylori cultures were positive in the same 3 patients, to whom 16SrRNA gene and cagA gene were positive. Because H.pylori exists not only in gastric mucosa but also in peripheral blood,it is possible that H. pylori can result in bacteremia.