| 高压氧对小鼠肺毛细血管Na~＋－K~＋－ATPase影响的图像分析 |
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| 引用本文： | 徐根兴,白志诚,汤先忻,张云鹏,袁驾南,董文度,张留保.高压氧对小鼠肺毛细血管Na~＋－K~＋－ATPase影响的图像分析[J].中国病理生理杂志,1996(2). |
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| 作者姓名： | 徐根兴 白志诚 汤先忻 张云鹏 袁驾南 董文度 张留保 |
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| 作者单位： | 南京海军医学高等专科学校 |
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| 基金项目： | 中国人民解放军总后科研基金 |
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| 摘 要： | 采用Ｎａ￣＋－Ｋ￣＋－ＡＴＰａｓｅ电镜细胞化学方法，观察在高压氧（０．２５ＭＰａ）和高压氧中毒情况下（０．５２ＭＰａ）小鼠肺毛细血管Ｎａ￣＋－Ｋ￣＋－ＡＴＰａｓｅ活性的分布、活性强度与超微结构改变之间的联系，并用计算机图像定量分析方法对酶活性进行了１４个参数的定量分析，这些定量参数表明，高压氧条件下。肺毛细血管Ｎａ￣＋－Ｋ￣＋－ＡＴＰａｓｅ活性升高，在高压氧中毒（０．５２ＭＰａ）条件下，肺毛细血管Ｎａ￣＋－Ｋ￣＋－ＡＴＰａｓｃ活性减弱。
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| 关 键 词： | 腺苷三磷酸酶类，钠，钾，酶类，高压氧，肺 |
Effects of hyperbaric oxygen on Na -K -AThase activities in mouse lung capillaries |
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| Xu Gen-Xing, Bai Zi-Cheng, Tang Xian-Xin, Zhang Yun-Peng,Yuan Jia- Nan, Dong Wen-Du, Zhang Liu-Bao.Effects of hyperbaric oxygen on Na -K -AThase activities in mouse lung capillaries[J].Chinese Journal of Pathophysiology,1996(2). |
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| Authors: | Xu Gen-Xing Bai Zi-Cheng Tang Xian-Xin Zhang Yun-Peng Yuan Jia- Nan Dong Wen-Du Zhang Liu-Bao |
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| Abstract: | The relationships between the localization and changes of Na -K -ATPase and theChanges of ultrastructure in mouse lung capillaries in hyperbaric oxygen(0.25MPa) and hyperbaricoxygen intoxication (0.52MPa) were studied using the ultra-cytochemical methods of Na -K -ATPase.A quantiative analysis of 14 parameters of the enzymative activities was made using animage analyser.The results showed that the activities of Na -K -ATPase were apparently increasedin hyperbaric oxygen(0.25MPa) and were decreased in hyperbaric oxygen intoxication( 0.52MPa). |
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