| 白蒲黄片质量标准的研究 |
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| 引用本文： | 李方,曹阳,张清波.白蒲黄片质量标准的研究[J].中医药信息,2010,27(3):60-62. |
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| 作者姓名： | 李方 曹阳 张清波 |
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| 作者单位： | 1. 黑龙江省药品检验所,黑龙江,哈尔滨,150001
2. 大庆市药品检验所,黑龙江,大庆,163316 |
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| 基金项目： | 国家药典委员会2010年版《中国药典》科研基金 |
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| 摘 要： | 目的:建立白蒲黄片的质量标准。方法:采用TLC法对处方中黄芩、黄柏进行了专属性鉴别,并用HPLC法对处方中黄芩进行了含量测定,采用C18色谱柱,以甲醇-水-磷酸(43∶57∶0.2)为流动相,流速为1.0mL.min-1,检测波长为280nm。结果:薄层色谱斑点清晰,易于识别,专属性强;黄芩苷线性范围为0.04420μg～0.442μg(r=1.0000),平均加样回收率为102.3%,RSD为1.5%。结论:本方法操作简便,快速、准确、灵敏度高,重复性好,提高后的质量标准能更有效的控制该制剂的质量。
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| 关 键 词： | 白蒲黄片：质量标准 薄层色谱 黄芩苷 高效液相色谱 |
Studies on Quality Standard of Niuhuang Qinggong Wan |
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| LI Fang,CAO Yang,ZHANG Qing-bo.Studies on Quality Standard of Niuhuang Qinggong Wan[J].Information on Traditional Chinese Medicine,2010,27(3):60-62. |
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| Authors: | LI Fang CAO Yang ZHANG Qing-bo |
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| Institution: | LI Fang1,CAO Yang2,ZHANG Qing -bo1 (1. Heilongjiang Provincial Institute for Drug Control,Harbin 150001,China,2. Daqing Provincial Institute for Drug Control,China) |
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| Abstract: | Objective：To determine the quality standard of Baipuhuang Tablets. Methods：Radix Scutellariae,Cortex Phellodendri Chinensis in Baipuhuang Tablets were identified by TLC. The content of Baicalin was determined by HPLC. Using ODS column,Methanol-Water -Phosphoric acid （43∶ 57∶ 0. 2） as the mobile phase at flow rate of 1. 0mL.min -1,wavelength of the detection was 280nm. Results：The qualitative identification with TLC were specific. liner range of Baicalin was 0. 0442μg ～ 0. 442μg（r = 1. 0000）;the recovery was 102. 3% （RSD = 1. 5% ）. Conclusion：This method is simple,rapid,accurate,highly sensitive and well re-producible. This method can be used for the quality control of Baipuhuang Tablets. |
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| Keywords: | Baipuhuang Tablets Quality control TLC Baicalin HPLC |
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