帕病2 号方对帕金森病模型大鼠黑质神经元形态学的影响

目的：探讨帕病2号方对帕金森病（PD）模型大鼠中脑黑质神经元的保护作用。方法：采用6-羟基多巴胺（6-OHDA）左侧纹状体两点注射法建立PD大鼠模型，经阿扑吗啡（APO）诱导表现恒定右侧旋转且30 min内旋转速度大于7 r·min-1者，视为成功PD大鼠模型。14只PD大鼠随机分为模型组、治疗组，同时设立正常组、假手术组。正常组、假手术组、模型组给予等容积蒸馏水，治疗组给予帕病2号方（32.0 g·kg-1），连续灌胃给药4周。通过Nissl染色及免疫组化方法检测中脑黑质神经元Nissl小体的变化及酪氨酸羟化酶（TH）、核因子E2相关性因子2（Nrf2）、血红加氧酶1（HO-1）的变化。结果：与正常组比较，模型组PD大鼠黑质神经元Nissl小体严重损伤，TH阳性细胞数目显著减少（P<0.01），Nrf2核蛋白、HO-1蛋白表达显著增强（P<0.01）。与模型组比较，帕病2号方治疗组能明显增加PD大鼠黑质神经元内Nissl小体数量，增加TH阳性细胞数目（P<0.05），同时明显上调Nrf2核内表达及HO-1蛋白的表达（P<0.05）。结论：帕病2号方干预对PD大鼠模型黑质神经元具有明显保护作用，可能与上调Nrf2核内表达和HO-1表达有关。

Effect of Pa-Bing Formula No. 2 on Morphological Changes of Subst antia Nigra Cells in Parkinson's Disease Rats

This article was aimed to study the protection effects of Pa-Bing Formula No. 2 (PBFN-2) on neurons of substantia nigra in Parkinson's disease (PD) rats models in order to explore the possible mechanism of PBFN-2 in PD treatment. Rats were stereotaxically injected with 6-hydroxydopamine (6-OHDA) solution into the left striatum in two-site. Rat showed consistent right whirling and the number of rotation was more than 7 r·min-1 induced by APO in 30 min, then the rat was judged as PD model. A total of 14 rats modeled successfully were randomly divided into the model group and the treatment group. At the same time, the normal group and sham operation group were also established. Same volume of distilled water was given to the normal group, sham operation group and the model group. PBFN-2 (32.0 g·kg-1) was given to the treatment group for 4 weeks. Microscope was used to observe pathological changes of substantia nigra by nissl stain and changes of TH, Nrf2 and HO-1 immunohistochemical stain. The results showed that compared with the normal group, the nissl bodies were badly injured. Expressions of TH-positive cells were obviously reduced (P<0.01). The expression of Nrf2 nucleus protein and HO-1 protein were obviously increased in substantia nigra of PD rats in model group (P<0.01). Compared to the model group, PBFN-2 effectively increased nissl bodies in neuronal cells of substantia nigra of PD rats, and elevated the number of THimmunoreactive cells in substantia nigra (P<0.05). The expressions of Nrf2 nucleus protein, HO-1 protein were significantly up-regulated (P<0.05). It was concluded that PBFN-2 had an obvious neuroprotection on the neuronal cells in substantia nigra of PD rats induced by 6-OHDA. The underlying mechanisms may be associated with regulation of Nrf2 nucleus protein and HO-1 protein expressions.