| Abstract: | Enzymolytic product (EP) of normal human plasma (NHP), was prepared through combined digestion of alpha-chymotrypsin and pepsin. This product was studied with MGc 80-3 cells (human gastric carcinoma cell line) and FC cells (615-murine gastric carcinoma cell line). The adhesive rate of NHP to MGc 80-3 cells may reach 90%, while the capacity of NHP-EP to inhibit adhesion reached over 90%, being specific and non-cytotoxic. Intravenous coinjection of NHP-EP with FC murine gastric carcinoma cells remarkably inhibited the formation of lung tubercles in 615-mice, with an inhibitory rate of 83.3% and a median survival time prolonged 35 days, which was only 19 days in the controls. The results showed that NHP-EP can inhibit experimental hematogenous metastasis through interfering the adhesion of tumor cells to extracellular stroma. We believe that NHP-EP may have practical value in preventing, seeding of metastatic cells after surgical removal of a primary tumor. |
