大孔树脂分离纯化刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的工艺研究

目的研究大孔树脂分离纯化刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的工艺。方法比较了4种不同类型的大孔树脂对刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的吸附解吸性能，确定适宜的树脂类型和最佳纯化工艺条件。结果AB-8型大孔树脂对紫丁香苷、刺五加苷E和异嗪皮啶的吸附性能及解吸效果较好。最佳工艺条件为上样浓度：0．5g·mL^-1，吸附流速：2BV·h^-1，上样量：5BV，洗脱剂：30％的乙醇，用量：11BV，洗脱流速：1BV·h^-1。纯化后的终产品中紫丁香苷、剌五加苷E和异嗪皮啶的含量分别为3．13％，1．82％和0．42％。结论AB-8型大孔树脂用于同时富集紫丁香苷、刺五加苷E和异嗪皮啶效果最佳，是一种理想的分离纯化介质。

Study on Separation and Purification of Syringin, Aleutheroside E and Isofraxidin from Acanthopanax Senticosus by Macroporous Resin

ABSTRCT: OBJECTIVE To study the separation and purification technology of syringin, eleutheroside E and isofraxidin from Acanthopanax Senticosus by macroporous resin. METHODS The adsorption and desorption rate of four types of macroporous resins was compared to determine the proper kind of resin and the best condition for purifying. RESULTS The adsorption and isolation effect of AB-8 type resin was much better. The optimal conditions were as: the concentration of sample solution was 0.5g·mL-1, the adsorption flow rate was 2BV·h-1 the maximum loading amount was 5BV, the eluent was 30% ethanol, the dosage was 11BV and the elution flow rate was 1BV·h-1. CONCLUSION AB-8 is an ideal resin with the best enrichment for separating and purifying syringin, eleutheroside E and isofraxidin simultanously.