不同栽培居群广藿香的种内遗传多样性研究

目的:探讨广藿香不同栽培居群的遗传多样性和种内分化水平,寻找有效地鉴定不同居群广藿香DNA指纹特征方法。方法:从80个随机引物中筛选出14个具较高多态性检测能力的引物。用这14个随机引物对5个栽培居群的广藿香进行了的分析,对得出的结果应用PopGen32软件进行计算和聚类分析。结果:实验测出84个位点,其中单态位点17个,占20.24%;多态位点67个,占79.76%。分析结果表明,同一种内不同栽培居群间存在明显的遗传分化。结论:应用RAPD选择特定引物对广藿香遗传多样性的分析及不同居群的鉴定是可行的。RAPD技术为广藿香遗传种质资源的鉴定和分类提供了新的途径。

Study on intraspecific genetic diversity in different plantpopulations of Pogostemon cabli

Objective: To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cabli,and find out the effective method to distinguish DNA fingerprint of different populations of P.cablin.Method: Five plant populations of P.cablin were analyzed by RAPD markers.PopGen 32 software for clustering analysis and calculating.Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character.Result: A total of 84 bands was amplified by the 10 primers,among them 17 bands were monomorphic.67 of them were polymorphic.The results indicated that the genetic variations existed within the different plant populations of the same species.Conclusion: It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P.cablin.RAPD technique has provided a new path for identification and classification of P.cablin genetic germplasm.