New HLA–DR haplotypes containing the DRB6 pseudogene |
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| Authors: | M Salazar C B Granja A Selvakumar B Fresia S Alosco B Dupont E J Yunis |
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| Institution: | American Red Cross, New England Region, Dedham, Massachusetts New York, USA;Dana-Farber Cancer Institute, Boston, Massachusetts New York, USA;Harvard Medical School, Boston, Massachusetts New York, USA;Sloan-Kettering Institute for Cancer Research, New York, New York, USA |
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| Abstract: | An unexpected probe reaction pattern was observed in two samples during HLA-DR typing by PCR-Sequence Specific Oligonucleotide Probes. In order to confirm the unusual typings, samples were analyzed by PCR-Sequence Specific Primers, cloning, and nucleotide sequencing of the second exon of the HLA-DRB-genes. The confirmed DR, DQ phenotype for one sample was DRB1*0701, DRB4*01, DRB5*0101, DRB6*0201, DQB*0602, DQB1*0202. The phenotype of other sample was DRB1*1602, DRB1*1302, DRB3*0301, DRB6*0101, DQB1*0501, DQB1*0502. The first sample has the novel combination of DRB1*0701 with DRB5*0101 and DRB6*0201. The second sample has either DRB6*0101 together with DRB 1*1602 in absence of any DRB5 allele or DRB6*0101 together with DRB1*1302, DRB 3*0301. We postulate that the most likely haplotype in sample #1 is DRB1*0701, DRB5*0101, DRB1*0602 which could have arisen from gene conversion. The most likely haplotype in sample #2, DRB 1*1602, DRB6*0101, DQB1*0502 would have arisen from an homologous recombination event. |
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| Keywords: | HLA-DR-DRB6 |
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