| 高效液相色谱法测定山豆根中高丽槐素的含量 |
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| 引用本文： | 黄韵诗. 高效液相色谱法测定山豆根中高丽槐素的含量[J]. 中南药学, 2008, 6(2): 193-195 |
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| 作者姓名： | 黄韵诗 |
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| 作者单位： | 浏阳市人民医院药剂科,长沙,410300 |
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| 摘 要： | 目的建立测定山豆根药材中高丽槐素的含量测定方法。方法色谱柱为KromasilODS-1（4.6mm×250mm,5μm）,流动相为乙腈-0.1%醋酸水溶液,梯度洗脱,流速为1.0mL·min^-1,检测波长为310nm,柱温为25℃。结果高丽槐素在0.1908～5.724μg线性关系良好,r=0.9999,平均回收率为100.4%,RSD=1.6%。结论该方法简便、准确、重复性好,提供了更好的控制山豆根药材质量的方法。
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| 关 键 词： | 山豆根 高丽槐素 高效液相色谱法 含量测定 |
| 文章编号： | 1672-2981(2008)02-0193-03 |
| 修稿时间： | 2008-01-25 |
Determination of maackiain in Sophora tonkinensis Gapnep.by HPLC |
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| HUANG Yun-shi. Determination of maackiain in Sophora tonkinensis Gapnep.by HPLC[J]. Central South Pharmacy, 2008, 6(2): 193-195 |
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| Authors: | HUANG Yun-shi |
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| Affiliation: | HUANG Yun-shi (Department of Pharmacy, Peoples Hospital of Liuyang , Changsha 410300) |
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| Abstract: | Objective To establish a method for the determination of maackiain in Sophora tonkinensis Gapnep.. Methods The separation was performed on a Krornasil OD-1 （4.6 mm×250 mm, 5μm）. The mobile phase consisted of ACN-0. 1% HAC, gradient elutve. The flow rate was 1, 0 mL · min^-1. The UV detective wavelength was 310 nm. Results The calibration curves of maackiain were in good linearity over 0. 190 8-5. 724μg （r=0. 999 9）. The average recovery was 100. 4% with RSD of 1.6%. Conclusion This method is simple, accurate and reproducible. It provides a better quality control method for Sophora tonkinensis Gapnep. |
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| Keywords: | Sophora tonkinensis Gapnep. maackiain HPLC content deterlnination |
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