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rhGDNF的抗癎作用与CaBPs上调的关系
引用本文:程庆璋,包仕尧,董万利. rhGDNF的抗癎作用与CaBPs上调的关系[J]. 中国临床神经科学, 2005, 13(3): 245-248
作者姓名:程庆璋  包仕尧  董万利
作者单位:1. 苏州第二人民医院,神经内科,215002
2. 苏州大学附属第二医院,神经内科,215004
摘    要:目的探讨大鼠致癎后海马区钙结合蛋白(CaBPs)的变化以及rhGDNF抗癎作用与CaBPs的关系.方法应用免疫组化法检测海人藻酸(KA)致癎前后以及rhGDNF干预后海马区3种CaBPs(PV、CR、CBN)的表达.结果KA致癎后大鼠海马区含3种CaBPs的GABA中间神经元明显下降,其中以CR下降显著,下降明显区域为齿状回(DG)区和门区;而rhGDNF干预后未见癎性发作,其海马各区CV、CR、CBN标记数较对照组增加.结论癫癎持续发作使海马区含3种CaBPs的GABA能中间抑制神经元丧失,而外源性rhGDNF具有抗癎样作用,其机制可能与增加海马区CaBPs有关.

关 键 词:癫癎  海马  钙结合蛋白  人类重组的胶质细胞源性神经营养因子  免疫组织化学
文章编号:1008-0678(2005)03-0245-04
修稿时间:2004-10-26

The Anti-epileptic Effects of rhGDNF and CaBPs Upregulation
Cheng Qing-zhang,BAO Shi-yao,Dong Wan-li. The Anti-epileptic Effects of rhGDNF and CaBPs Upregulation[J]. Chinese Journal of Clinical Neurosciences, 2005, 13(3): 245-248
Authors:Cheng Qing-zhang  BAO Shi-yao  Dong Wan-li
Abstract:Aim:To investigate the changes of CaBPs expression in GABA interneurons in hippocampus of epileptic rats and to explore the relationship between rhGDNF anti-epileptic effects and CaBPs. Methods:Three kinds of CaBPs (PV,CR,CBN) in hippocampus were examined by immunohistochemical method in the pre,post KA-induced epileptic rats and rhGDNF treated rats.Results:In KA-induced epilepsy model group,the number of GABAergic interneuron containing CaBPs( PV,CR,CBN) was markedly decreased especially CR positive neurons compared to the control group,and the most apparently decreased regions were dentate gyrus (DG) and hilum. There was no seizure attack in treated group with rhGDNF and the positive neurons for PV,CR,CBN in the group were more than that in the control.Conclusion:Status epilepticus(SE) could cause the loss of hippocampus GABA interneurons which contains CaBPs. The mechanism of exogenous rhGDNF anti-epileptic effects may be associated with the increasing of hippocampal CaBPs.
Keywords:Epilepsy  Hippocampus  Calcium-binding proteins(CaBPs)  Recombinant human Glial cell line-derived neurotrophic factor (rhGDNF)  Immunohistochemistry
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