rhGDNF的抗癎作用与CaBPs上调的关系

目的探讨大鼠致癎后海马区钙结合蛋白(CaBPs)的变化以及rhGDNF抗癎作用与CaBPs的关系.方法应用免疫组化法检测海人藻酸(KA)致癎前后以及rhGDNF干预后海马区3种CaBPs(PV、CR、CBN)的表达.结果KA致癎后大鼠海马区含3种CaBPs的GABA中间神经元明显下降,其中以CR下降显著,下降明显区域为齿状回(DG)区和门区;而rhGDNF干预后未见癎性发作,其海马各区CV、CR、CBN标记数较对照组增加.结论癫癎持续发作使海马区含3种CaBPs的GABA能中间抑制神经元丧失,而外源性rhGDNF具有抗癎样作用,其机制可能与增加海马区CaBPs有关.

The Anti-epileptic Effects of rhGDNF and CaBPs Upregulation

Aim:To investigate the changes of CaBPs expression in GABA interneurons in hippocampus of epileptic rats and to explore the relationship between rhGDNF anti-epileptic effects and CaBPs. Methods:Three kinds of CaBPs (PV,CR,CBN) in hippocampus were examined by immunohistochemical method in the pre,post KA-induced epileptic rats and rhGDNF treated rats.Results:In KA-induced epilepsy model group,the number of GABAergic interneuron containing CaBPs( PV,CR,CBN) was markedly decreased especially CR positive neurons compared to the control group,and the most apparently decreased regions were dentate gyrus (DG) and hilum. There was no seizure attack in treated group with rhGDNF and the positive neurons for PV,CR,CBN in the group were more than that in the control.Conclusion:Status epilepticus(SE) could cause the loss of hippocampus GABA interneurons which contains CaBPs. The mechanism of exogenous rhGDNF anti-epileptic effects may be associated with the increasing of hippocampal CaBPs.