| 羊栖菜多糖对SGC-7901人胃癌细胞内[Ca2+]i的影响 |
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| 引用本文： | 高世勇,季宇彬. 羊栖菜多糖对SGC-7901人胃癌细胞内[Ca2+]i的影响[J]. 天津中医药, 2003, 20(4): 62-64 |
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| 作者姓名： | 高世勇 季宇彬 |
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| 作者单位： | 哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨,150076 |
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| 基金项目： | 国家自然科学基金资助课题 (30271598)，教育部科技研究重点项目 (01035)，黑龙江省普通高等学校骨干教师创新能力计划 (2002015)，黑龙江省自然科学基金(D00 -18) |
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| 摘 要： | [目的]观察羊栖菜多糖对人胃癌细胞内Ca^2 含量的变化，揭示其诱导肿瘤细胞凋亡的机制。[方法]采用Fluo-3／AM探针标记，激光共聚焦技术观测细胞内[Ca^2 ]i。[结果]SFPS可使SGC-7901细胞内[Ca^2 ]i先升高然后下降，给CaCl2后，[Ca^2 ]i又升高。[结论]SFPS诱导肿瘤细胞凋亡是通过升高SGC-7901细胞内[Ca^2 ]i而达到的，[Ca^2 ]i升高时Ca^2 来源于细胞内钙库释放。
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| 关 键 词： | 羊栖菜多糖 SGC-7901 人胃癌 癌细胞内[Ca^2＋]i 细胞凋亡 |
| 文章编号： | 1672-1519(2003)04-0062-03 |
| 收稿时间： | 2003-03-13 |
| 修稿时间： | 2003-03-13 |
Influence of SFPS on [Ca2+]i of SGC-7901 |
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| GAO Shi-yong and JI Yu-bin. Influence of SFPS on [Ca2+]i of SGC-7901[J]. Tianjin Journal of Traditional Chin Medicine, 2003, 20(4): 62-64 |
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| Authors: | GAO Shi-yong and JI Yu-bin |
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| Affiliation: | Postdoctoral Programme, Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China;Postdoctoral Programme, Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China |
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| Abstract: | ObjectiveTo study the change of Ca2+ in SGC-7901, for exploring the mechanism of apoptosis induced by SPFS.Intracellulari was measured with laser confocal microscopy using Fluo-a/Amoribe labeling technique.SFPS, increased i first and then declined. After giving CaCl2, i rise again. [Conclusion]SFPS can induce apoptosis of cancer cells through enhancing i in SGC-7091. This kind of rises of Ca2+ is from the release of cellular storage. |
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| Keywords: | SFPS SGC-7901 i action of effect |
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