53BP2 induces apoptosis through the mitochondrial death pathway |
| |
Authors: | Kobayashi Shinya Kajino Shinichi Takahashi Naoko Kanazawa Satoshi Imai Kenichi Hibi Yurina Ohara Hirotaka Itoh Makoto Okamoto Takashi |
| |
Affiliation: | Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi 467-8601, Japan. |
| |
Abstract: | The p53 binding protein 2 (53BP2) has been identified as the interacting protein to p53, Bcl-2, and p65 subunit of nuclear factor kappaB (NF-kappaB). The TP53BP2 gene encodes two splicing variants, 53BP2S and 53BP2L, previously known as apoptosis stimulating protein 2 of p53 (ASPP2). We found that these 53BP2 proteins are located predominantly in the cytoplasm and induce apoptosis as demonstrated by cleavage of poly ADP ribose polymerase (PARP) and annexin V staining. Furthermore, we demonstrate that 53BP2 is located in the mitochondria and induces apoptosis associated with depression of the mitochondrial trans-membrane potential (DeltaPsim) and activation of caspase-9. From these findings we conclude that 53BP2 induces apoptosis through the mitochondrial death pathway. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|