Increase of reactive oxygen (ROS) and nitrogen (RNS) species generated by phagocyting granulocytes related to age |
| |
Authors: | Martins Chaves M Rocha-Vieira E Pereira dos Reis A de Lima e Silva R Gerzstein N C Nogueira-Machado J A |
| |
Affiliation: | Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Ant?nio Carlos 6627 CP 486, 30161-970, Minas Gerais, Belo Horizonte, Brazil. chavesmm@mono.icb.ufmg.br |
| |
Abstract: | In this present paper the age-induced effect on reactive oxidizing species generated by oxygen (ROS) and nitrogen (RNS) was studied using human phagocyting granulocytes. The ROS and RNS were quantified, respectively, in a chemiluminescence assay and by the measurement of nitrite production. The age-induced reactive oxidizing species generation was studied in healthy subjects ranging from 20 to 80 years old, divided into six age groups: group I, 20-29 years old; group II, 30-39 years old; group III, 40-49 years old; group IV, 50-59 years old; group V, 60-69 years old; and group VI, 70-80 years old. Our results demonstrate a parallelism between generation of the ROS and RNS induced by the age. A significant increase of ROS production was observed from 40 years old (age groups III, IV, V and VI while for RNS this increase was observed only from 50 years old (groups IV, V and VI). These data suggest an increase of oxidizing species generation (ROS/RNS) related to age. The increased generation of ROS (40-49 years old) was induced before the increasing of RNS (50-59 years old) and it may have consequences on inflammation and host defences. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|