中药脊髓I号对脊髓损伤大鼠背根节CGRP表达的影响

为研究在脊髓损伤后服用中药脊髓I号 (SCI)对背根节 (DRG)神经元降钙素基因相关肽 (CGRP)表达的影响 ,探讨SCI促进脊髓修复再生效应的机制 ,用免疫细胞化学法和定量图像分析法 ,检查了SCI对Wistar大鼠下胸髓半横断损伤诱发的局部DRG神经元CGRP表达的影响。结果发现 :①脊髓半横断引起同侧首、尾向各 3个DRG内CGRP表达的明显和持续性下调 ;②损伤后投给SCI冲剂 ,可以明显减轻、但不能完全阻止脊髓损伤诱发的CGRP表达下调 ;③损伤后投给中药补阳还五汤 ,或用大剂量的氢化可的松治疗 ,不能减轻脊髓损伤诱发的CGRP表达下调 ;④在服用SCI大鼠脊髓的损伤区 ,显示有明显的修复再生。提示 :CGRP表达程度与脊髓损伤及其修复再生的情况相关 ;SCI可能通过对CGRP mRNA基因表达的调节 ,激发损伤脊髓组织的修复再生。

Effect of Chinese Herb Spinal Cord Granule I on CGRP Expression in Located Dorsal Root Ganglia in Rats with Spinal Cord Hemisection

In order to study effect of spinal cord granule I (SCI) on calcitonin gene related peptide (CGRP) expression in dorsal root ganglia (DRG) after spinal cord injury and to inquire into mechanism of promoting spinal cord regeneration in rats with SCI, effect of SCI on CGRP expression in DRG of Wistar rats subjected to right hemisection at the lower thoracic spinal cord were evaluated by immunocytochemistry and quantitative image analysis. The results are followed as: ① A significant down regulation of CGRP expression in ipsilateral 3 DRG oral and caudal to the injury level was caused by spinal cord hemisection respectively. The down regulation of CGRP expression is consistent and no reversed during one month after spinal cord injury. ② The down regulation of CGRP expression was evidently relieved but not prevented with administration of SCI. ③ The down regulation of CGRP expression induced by spinal cord injury was not relieved with administration of Buyang huanwutang or hydrocortisone in a large doses. ④The spinal tissue injured was repaired and regenerated in rats with administration of SCI. So that, the results not only suggested a possibility that reversion of CGRP expression following down regulation may be as a mark for regeneration of spinal cord tissue injured, but also indicated that it may be one of mechanisms for promoting repair and regeneration of spinal cord injured that SCI regulates CGRP mRNA expression.