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| 人类疱疹病毒-2对HaCaT细胞表达GCSF和GMCSF的影响 | |
| 引用本文： | 邵勇,钟绮丽,万峻,张杰,张伟,于波.人类疱疹病毒-2对HaCaT细胞表达GCSF和GMCSF的影响[J].中国中西医结合皮肤性病学杂志,2010,9(2):97-99. |
| 作者姓名： | 邵勇 钟绮丽 万峻 张杰 张伟 于波 |
| 作者单位： | 1. 北京大学深圳医院,深圳,518036 2. 深圳北京大学香港科技大学医学中心,深圳,518036 3. 暨南大学药学院,广州,510632 |
| 摘 要： | 目的探讨HaCaT细胞在人类疱疹病毒-2(HSV-2)刺激前后粒细胞集落刺激因子(GCSF)和粒/巨噬细胞集落刺激因子(GMCSF)的表达变化及意义。方法以VERO细胞进行HSV-2扩增,采用RT-PCR和荧光实时定量PCR检测HSV-2感染HaCaT细胞前及感染后24h、48h、72h时GCSF和GMCSF的表达量。结果HaCaT细胞可自分泌GCSF和GMCSF;HSV-2感染后24h,HaCaT细胞表达GCSF及GMCSF的水平增高,72h时又明显上升,且实验组与对照组相比两种细胞因子的表达有显著性差异。结论HaCaT细胞可以自发表达GCSF和GMCSF;HSV-2刺激对HaCaT细胞表达GCSF和GMCSF有促进作用。 |
| 关 键 词： | HaCaT细胞 人类疱疹病毒-2 粒细胞集落刺激因子 粒/巨噬细胞集落刺激因子 |
Expression of Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor Beforeand After HaCaT Cell Infection with HSV-2 |
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| SHAO Yong,ZHONG Qi-li,WAN Jun,ZHANG Jie,ZHANG Wi,YU Bo.Expression of Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating Factor Beforeand After HaCaT Cell Infection with HSV-2[J].Chinese J of Dermatovenerology Integr Tradit and West Med,2010,9(2):97-99. | |
| Authors: | SHAO Yong ZHONG Qi-li WAN Jun ZHANG Jie ZHANG Wi YU Bo |
| Institution: | SHAO Yong1,ZHONG Qi-li1,WAN Jun2,ZHANG Jie1,ZHANG Wei3,YU Bo1 1. Shenzhen Hospital of Peking University,Shenzhen 518036,China,2. Shenzhen-PKU-HKUST Medical Center,3. Jinan University,Guangzhou 510632 |
| Abstract: | Objective To investigate the expression of granulocyte colony-stimulating factor (GCSF) and granulocyte-macrophage colony-stimulating factor (GMCSF) before and after HaCaT cell infection with human herpes virus (HSV)-2. Methods The virus were amplified and titrated by using VERO cells. RT-PCR and Real-time quantitative PCR was employed to determine the expression of GCSF and GMCSF in the HaCaT cells infected by HSV-2. Results GCSF and GMCSF was expressed in the HaCaT cells. After HSV-2 infection, the expres... |
| Keywords: | HaCaT cell human herpes virus-2 granulocyte colony-stimulating factor granulocyte-macrophage colony-stimulating factor |
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