大鼠脑缺血损伤后MDA含量和SOD活性变化及胡黄连苷Ⅱ干预作用

目的通过正交试验优化胡黄连苷Ⅱ治疗大鼠脑缺血损伤的最佳剂量和时间窗。方法应用双侧颈总动脉结扎法(BCCAO)建立大鼠前脑缺血模型,按照正交试验设计分组,经腹腔注射胡黄连苷Ⅱ干预治疗,硫代巴比妥酸法测定血清和脑组织中脂质过氧化物丙二醛(MDA)的含量。黄嘌呤氧化酶法检测血清和脑组织中超氧化物歧化酶(SOD)的活性。结果胡黄连苷Ⅱ治疗脑缺血损伤的最佳效果,根据血清和脑组织MDA含量分析,均为脑缺血1.5h腹腔注射10mg/kg体质量。根据血清和脑组织SOD活性分析,均为脑缺血1.5h腹腔注射20mg/kg体质量。结论从用药剂量最小化和治疗时间窗最大化的角度综合评价,胡黄连苷Ⅱ治疗脑缺血损伤的最佳治疗时间窗和剂量为脑缺血1.5 h腹腔注射10-20mg/kg体质量。

The Effect of Picroside II on the Concentration of NO and the Activity of GSHPx after Cerebral Ischemic Injury in Rats

Objective To optimize the therapeutic dose and time window of picrosede rl in cerebral ischemic injury in rats by orthogonal test.Methods The forebrain ischemia models were established by bilateral common carotid artery occlusion （BCCAO） methods.The successful models were randomly grouped according to orthogonal experimental design and treated by injecting picroside I1 intraperitonenally at different ischemic time with different dose.The concentration of lipid perioxide malondialdehyde （MDA） in serum and tissue of was determined by thiobarbituric acid assay.The activity of superoxide dismutase （SOD） in serum and brain tissue was determined by xanthine oxidase assay.Result The optimized composition of the therapeutic dose and time window of picroside II in cerebral ischemic injury was ischemia 1.Sh with 10mg/kg body weight according to the concentration of MDA in serum and brain tissue, ischemia 1.5h with 20mg/kg body weight according to the activity of SOD in serum and brain tissue. Conclusion From the principle of lowest therapeutic dose with longest tome window, the optimized composition of the therapeutic dose and time window of picroside II in cerebral ischemic injury is ischemia 1.5h with 10-20mg/kg body weight.