抗白念珠菌芽管单克隆抗体杂交瘤细胞株的建立及其对血清芽管诱导率的影响

目的制备并鉴定抗白念珠菌芽管单克隆抗体杂交瘤细胞株,观察该单抗对白念珠菌菌相转换的影响。方法白念珠菌ATCC-90028标准株带芽管孢子做抗原,运用细胞融合技术,制备抗白念珠菌芽管胞壁外膜抗原单克隆抗体杂交瘤细胞株,并鉴定。将该单抗参与白念珠菌芽管血清诱导,对比其芽管诱导率与血清芽管诱导率。结果建立1株持续分泌抗白念珠菌芽管胞壁外膜抗原单克隆抗体的杂交瘤细胞株M cAb03.2C1-C2。其小鼠腹水单克隆抗体效价达1∶25 600;抗体重链属IgG1亚类,轻链属λ型;证实M cAb03.2C1-C2的靶位是位于白念珠菌芽管胞壁外膜上分子量为156kD的抗原成分。间接免疫荧光(IIF)可见单抗能与白念珠菌芽管特异性地结合,与白念珠菌的孢子相不发生反应。单抗能明显降低芽管诱导诱导率。结论本研究建立的M cAb03.2C1-C2对白念珠菌致病相-芽管有高度的特异性,为早期、快速地诊断系统性白念珠菌感染提供了新的前景。M cAb03.2C1-C2对菌相转换有明显的抑制作用,可能在抗白念珠菌系统性感染中起保护性作用。

Preparation and Identification of Monoclonal Antibody Against Candida albicans and the Influence Caused by McAb to Germ TubesInduction

Objective To develop and identify monoclonal antibodies against germ tubes of Candida albicans(C.albicans).Methods Blastospore of C.albicans ATCC-90028 were used as immunogens to prepare monoclonal antibodies(McABs),which were prepared by the lymphocyte hybridom technique,and were identificated.The result of germ tubes induction,which were induced by NCS,and with the paiticipation of McAb against C.albicans germ tubes,were observed.Results One celling of hybirdomase-creating constantly McAbs against germ tubes of C.albicans,which named McAb03.2C1-C2,was developed.The titer of McAb reached to 1∶25 600;The McAb belonged to IgG1 Lambda isotypes.The result that McAb03.2C1-C2 could specific react to the protein(156kD) antigen of germ tubes of Candida albicans was manifested.IIF diplaid that McAb03.2C1-C2 of mice hydroperitonia could specificly reacted to germ tubes of Candida albicans,but failed to react to phas of spore.Inductive radio of C.albicans germ tubes,which inducted by medium with the addition of McAb against C.albicans germ tube,was lower than inductive radio of C.albicans germ tube inducted by serum.Conclusion It is suggested that McAb03.2C1-C2 could be highly specific McAb against pathtype of Candida albicans-germ tubes,and it might be used in early and quick diagnoses of Systemic of C.albicans with a new prospect.McAb McAb03.2C1-C2,which could inhibited distinctly the diversion of C.albicans,can be a protective factor during the procress of resisting infection of systemic C.albicans.