左旋多巴对帕金森病动物模型抗氧化能力的影响

目的观察左旋多巴对帕金森病 (PD )模型大鼠的黑质抗氧化系统和线粒体呼吸链功能的影响 ,并探讨其作用机理。方法应用 6 OHDA制作PD大鼠模型。将 16只成功PD模型大鼠分为两组 (每组 8只 ) :模型组和左旋多巴 (L dopa)组 ,另设正常对照组 ( 8只 ) ,各组分别给予相应处理 ,共 45d ,给药前后分别进行行为学测试 ,给药后测定黑质区谷胱甘肽过氧化物酶 (GSH Px)、丙二醛 (MDA)、活性氧 (ROS)及线粒体呼吸链酶复合体Ⅰ水平。结果 ( 1)L dopa可使大鼠旋转行为明显改善 (P <0 .0 0 1)。 ( 2 )L dopa可降低GSH Px活性 ( 6.83± 0 .3 3 ) ,升高ROS、MDA水平 ( 68.44± 2 .3 4,2 .49± 0 .2 5 ) (与模型组比较P均 <0 .0 5 )。 ( 3 )L dopa组呼吸链酶复合体I明显低于模型组 ( P <0 .0 5 )。结论L dopa能有效改善旋转行为 ,但可加重黑质区氧化应激损伤 ,抑制线粒体呼吸链酶活性 ,提示长期应用L dopa制剂有可能加重DA能神经元的进行性缺失。

The effects of levodopa on antioxidation function of parkinson disease rat

ObjectiveTo observe the effects of levodopa on nigral antioxidation system and mitochondrial respiration-chain function, and study its mechanism.Methods The model rats for PD were established through 6-OHDA. The success 16 model rats were randomly divided into 2 groups:Model group(MG), levodopa group (LG), In addition, the normal group was set. Every group was given relevant treatment for 45 days. All rats behavior were tested before and after giving medicine . The activity of GSH-Px, contents of MDA, ROS and Mitochondrionase complex I of nigra were assayed after giving medicine .Results (1)LG decreased rotation behavior (P<0.01).(2)Levodopa reduced GSH-Px activity, improved ROS and MDA contents (all P<0.05). (3)Complex I content of LG decreased compared with MG (P<0.05). ConclusionL-dopa could obviously decrease rotation behavior, but aggravate oxidative stress of nigra, restrain the activity of mitochondrial respiration-chain,Which indicate that long-time taking L-dopa maybe aggravate the progressive lack of nigral dopaminergic neurons.