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血液分析仪白细胞分类不准确的因素
引用本文:舒美莲.血液分析仪白细胞分类不准确的因素[J].中国医疗前沿,2012(24):51-52.
作者姓名:舒美莲
作者单位:湖南省怀化市沅陵县中医院检验科
摘    要:目的探讨迈瑞血液五分类分析仪检测外周血细胞白细胞分类不准确的影响因素。方法运用迈瑞公司生产的BC-5800全自动五分类血液分析仪对外周血细胞进行自动分析,然后用手工涂片端氏染色进行显微镜手工分类。对两种方法的白细胞分类结果进行比对。结果全自动血液分析仪白细胞不准确包括两种情况:白细胞完全不分类;白细胞分类比例不正确。发现标本离体后立即上机进行检测,单核细胞的比例将会明显增高,有严重肝病、肾病、脂质代谢严重紊乱、新生儿等患者白细胞不分类的比例明显增高,经稀释后再上机,该白细胞分类与手工分类结果基本一致。重度病毒感染、核左移、血液病时所谓的单核细胞比例增高。经手工涂片染色分析,标本中的异淋、幼稚细胞、杆状核细胞都基本已被血液分析仪误认为单核细胞。放疗、化疗患者出于受射线、药物的影响,细胞的形态、结构也有所改变,血液分析仪白细胞分类也有较大误差。

关 键 词:迈瑞BC-5800全自动血细胞分析仪  白细胞分类不准确  手工涂片瑞氏染色分类

Blood analyzer white blood cell classification inaccurate factor
Abstract:Objective Explore MINDRAY five classifications of blood analyzers inaccurate detection of peripheral blood leukocyte classification factors. Method Use MINDRAY company produced BC-5800 automatic blood Analyzer in five categories of automatic analysis of the peripheral blood cells. Then microscopes with Hand smears Wright's staining Classification. Manual categorization. And the Leukocyte classification results of two methods for comparison. Results Leukocyte inaccurate automatic blood Analyzer includes two things: white blood ceils without classification, proportion of white blood cell. Found on the specimen immediately after in vitro testing, increased monocyte percentage would be significantly, and with severe liver disease, kidney disease, severe disorders of lipid metabolism, neonatal, Unsorted of white blood cells in patients with apparent increase. After dilution, then the machine testing, and the blood cell classification and manual classification results are basically the same. Nuclear left blood disease called severe virus infection increased monocyte percentage. After Hand smears analysis of dye, Specimens caught in the different cells, naive cells, rod cells are the basic blood Analyzer has been mistaken for monocytes. Patients undergoing radiotherapy and chemotherapy for patients affected by Ray, drug effects, changes in cell morphology, structure, Leukocyte classification by blood Analyzer will also have a greater margin of error.
Keywords:MINDRAY BC-5800 automatic blood ceil Analyzer  Leukocyte classification is inaccurate  Hand smears Wright' s staining Classifi-cation
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