| 早期糖尿病肾病患者亚甲基四氢叶酸还原酶C676T突变的相关因素及中医证型分析 |
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| 引用本文： | 林兰,郭小舟,倪青,龚燕冰,王斌,石世华.早期糖尿病肾病患者亚甲基四氢叶酸还原酶C676T突变的相关因素及中医证型分析[J].中医杂志,2010,51(5). |
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| 作者姓名： | 林兰 郭小舟 倪青 龚燕冰 王斌 石世华 |
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| 作者单位： | 1. 中国中医科学院广安门医院内分泌科,北京市宜武区北线阁
2. 北京王府中西医结合医院 |
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| 基金项目： | 国家自然科学基金资助项目(30672763) |
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| 摘 要： | 目的探讨早期糖尿病肾病(DN)患者基因突变与中医证型的关系。方法应用聚合酶链反应限制性片段长度多态性的方法,检测63例早期DN患者亚甲基四氢叶酸还原酶(MTHFR)基因的677碱基多态性情况,测定患者血浆同型半胱氨酸(Hcy)、叶酸浓度、空腹血糖、餐后血糖、糖化血红蛋白(HbA1C)、尿白蛋白排泄率(UAER)及血脂,并记录患者的中医证型。结果63例早期DN患者中CC型19例,TT例17例,CT型27例,TT、TC、CC的基因型频率分别为27.00%、42.85%、30.15%;T等位基因频率为48.41%,C等位基因频率51.59%。MTHFR基因的C676T突变与Hcy水平相关(P0.01);与甘油三酯(TG)水平相关(P0.05)。中医证型与基因型具有相关性(P0.01),CC型基因多辨证为阴虚热盛,杂合子基因型CT多辨证为气阴两虚,纯合子基因型TT多辨证为阴阳两虚。结论早期糖尿病肾病患者MTHFR基因的677碱基多态性与Hcy、TG、中医证候相关。
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| 关 键 词： | 糖尿病肾病 中医证候 亚甲基四氢叶酸还原酶 同型半胱氨酸 |
Analysis on the Correlated Factors of Methylene Tetrahydrofolate Reductase C676T Mutation and the Different TCM Syndromes in Early Diabetic Nephropathy |
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| LIN Lan,GUO Xiaozhou,NI Qing,GONG Yanbing,WANG Bin,SHI Shihua.Analysis on the Correlated Factors of Methylene Tetrahydrofolate Reductase C676T Mutation and the Different TCM Syndromes in Early Diabetic Nephropathy[J].Journal of Traditional Chinese Medicine,2010,51(5). |
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| Authors: | LIN Lan GUO Xiaozhou NI Qing GONG Yanbing WANG Bin SHI Shihua |
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| Institution: | LIN Lan1,GUO Xiaozhou1,NI Qing1,GONG Yanbing1,WANG Bin1,SHI Shihua2(1.Guang\'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,2.Beijing Royal Integrative Medicine Hospital) |
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| Abstract: | Objective To explore the relationship between gene mutation and different syndromes of TCM in the early stage of diabetic nephropathy(DN).Methods Sixty-three patients with diabetic nephropathy in the early stage were observed.The methylene tetrahydrofolate reductase(MTHFR)C676T polymorphism was determined by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP)assay.The levels of homocysteine(Hcy),acidum folicum,fasting glucose,postprandial glucose,glycosylated hemoglobin(HbA1C),urina... |
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| Keywords: | Diabetic nephropathy TCM syndrome Methylene tetrahydrofolate reductase(MTHFR) Homocysteine(Hcy) |
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