黄芪对脂多糖诱导大鼠急性肺损伤^p38 MAPK表达的影响

目的探讨脂多糖（LPS）诱导急性肺损伤（ALI）大鼠肺组织磷酸化p^38 MAPK表达的变化以及黄芪对其之影响。方法静脉注射LPS复制大鼠ALI模型。随机分成3组：正常对照组、ALI组和黄芪组。采用蛋白质印迹检测肺组织磷酸化p^38 MAPK的表达,并观察大鼠动脉血氧分压（PaO2）、肺湿/干重比（W/D）、支气管肺泡灌洗液（BALF）白蛋白、血清TNF-α的变化以及肺组织病理学改变。结果LPS诱导大鼠ALI时肺组织磷酸化p^38 MAPK的表达较正常对照组显著增加（P〈0.01）。黄芪注射液可显著抑制大鼠肺组织磷酸化p^38MAPK表达,降低W/D、BALF白蛋白以及血清TNF-α含量,使下降的PaO2回升,减轻肺组织病理学损伤。结论ALI时肺组织p^38 MAPK磷酸化表达增加;黄芪注射液对内毒素性肺损伤有保护作用,其机制可能与抑制磷酸化p^38 MAPK的表达有关。

Effect of Astragalus on the Expression of p38 Protein Kinase in Rats with Lipopolysaccharide-induced Acute Lung Injury

Objective To study the expression of p^38 protein kinase in LPS-induecd acute lung injury （ALI） and the intervention effect of astragalus. Methods LPS was injected intravenously to prepare ALI model. The rats were divided into three groups randomly： control group, LPS group and astragalus group. The expression of phospho-p^38 protein kinase was analyzed by western blot. Changes of Pa02, wet/dried lung weight（ W/ D）, albumin in BALF,TNF-α in serum and histopathology were observed. Results Resting rat lung expressed a little phospho-p^38; the expression of phospho-p^38 was up-regulated significantly in the lung after rats were injected with LPS （ P 〈 0.01）. Compared with LPS group, astragalus significantly inhibited the expression of phospho-p^38, decreased PaO2, W/D, albumin in BALF and TNF-α in serum （P 〈0.01 ）, and alleviated histropathologic damage alleviated. Conclusion The expression of phospho-p^38 is up-regulated in rat lung with LPS-induecd ALI. Astragalus injection has a protective effect on ALI and its therapeutic mechanism may be related to inhibition of the phosphorylation of p^38 MAPK.