ICA和PJA对高转移性人肺癌细胞体外侵袭转移能力抑制的研究

目的：将新型抗癌药物淫羊藿甙（ＩＣＡ）和济南假单胞菌制剂（ＰＪＡ）作用于ＰＧ细胞，从肿瘤转移抑制的多个方面和环节探讨ＰＪＡ、ＩＣＡ抗转移作用的机制。方法：采用粘附实验、运动侵袭实验、逆转录ＰＣＲ（ＲＴ－ＰＣＲ）、细胞免疫组化等多种方法进行了检测。结果：ＰＪＡ、ＩＣＡ可降低ＰＧ细胞对胞外基质的粘附性及侵袭、运动能力，减少ＰＧ细胞表面粘附分子ＣＤ４４Ｖ６、ＬＮ－Ｒ及胞浆内ＣＫ１８的表达，同时细胞内ｃ－ｍｙｃ、Ｔｉａｍ－１基因ｍＲＡＮ水平均有不同程度的降低，而Ｎｍ２３－Ｈ１ ｍＲＮＡ水平有不同程度的升高，且两药有明显的协同作用。结论：ＰＪＡ、ＩＣＡ通过对肿瘤转移多个步骤的抑制而发挥抗转移作用。

Studies of suppression on invasion and metastasis ability of high metastatic hum an lung tumor cell line

Objective: In order to further elucidate the anti-metastatic mechanism of PJA、ICA, PG cells were treated with PJA、ICA in vitro. Methods: In this study , adhesion assay, invasion assay, migration assay, RT-PCR technique and immun ohistochemical straining were used. Results:PJA、ICA could decrease PG cells adhesive ratio to laminin substrate and decreased the ability of invasion or migration. On the other hand, PJA、ICA reduced t he expression of CD44V6、LN-R and CK18 of PG cells. At the same time, th e relative expression levels of both c-myc and Tiam-1 mRNA decreased compared wi th that of the control. On the contrary, a tendency of the enhancement in the e x pression level of Nm23-H1 mRNA was also observed. Also found that PJA an d ICA had cooperative function . Conclusion: These results suggest that PJA、ICA may play anti-metastatic roles at many aspects.